Three-dimensional fabrication of thick and densely populated soft constructs with complex and actively perfused channel network

Acta Biomaterialia
Rodrigo Pimentel CMartin Dufva

Abstract

One of the fundamental steps needed to design functional tissues and, ultimately organs is the ability to fabricate thick and densely populated tissue constructs with controlled vasculature and microenvironment. To date, bioprinting methods have been employed to manufacture tissue constructs with open vasculature in a square-lattice geometry, where the majority lacks the ability to be directly perfused. Moreover, it appears to be difficult to fabricate vascular tissue constructs targeting the stiffness of soft tissues such as the liver. Here we present a method for the fabrication of thick (e.g. 1 cm) and densely populated (e.g. 10 million cells·mL-1) tissue constructs with a three-dimensional (3D) four arm branch network and stiffness in the range of soft tissues (1-10 kPa), which can be directly perfused on a fluidic platform for long time periods (>14 days). Specifically, we co-print a 3D four-arm branch using water-soluble Poly(vinyl alcohol) (PVA) as main material and Poly(lactic acid) (PLA) as the support structure. The PLA support structure was selectively removed, and the water soluble PVA structure was used for creating a 3D vascular network within a customized extracellular matrix (ECM) targeting the stiffness of the ...Continue Reading

Citations

Apr 11, 2018·Biomaterials Research·Janarthanan Gopinathan, Insup Noh
Apr 12, 2018·Biofabrication·Xinda LiTao Xu
Mar 28, 2019·Tissue Engineering. Part C, Methods·Bai YangNarutoshi Hibino
Feb 12, 2019·Biofabrication·Alisa Morss ClyneAndrés Díaz Lantada
Mar 4, 2020·Advanced Healthcare Materials·Jing NieYong He
Apr 3, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Hiroyuki Tetsuka, Su Ryon Shin
Aug 1, 2020·Small·Amin GhavamiNejadAli Khademhosseini
Jul 9, 2020·American Journal of Physiology. Cell Physiology·Chiara ScognamiglioGianluca Cidonio
Feb 23, 2020·Bioengineering·Andreas M A O Pollet, Jaap M J den Toonder
Oct 20, 2020·Advanced Healthcare Materials·Liang MaHuayong Yang
Mar 16, 2021·Journal of Biomaterials Science. Polymer Edition·Dilara Goksu Tamay, Nesrin Hasirci
Jun 1, 2021·Frontiers in Bioengineering and Biotechnology·Earnest P ChenJohn P Geibel
Jun 8, 2021·JACC. Basic to Translational Science·Kevin SungKim-Lien Nguyen
Jun 15, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Baosen TanXiaoming Li
Aug 6, 2021·Advanced Science·Alessandra DellaquilaTeresa Simon-Yarza
Aug 29, 2020·Chemical Reviews·Andrea SchwabJos Malda
May 15, 2020·Chemical Reviews·Carlos MotaLorenzo Moroni

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