Three-Dimensional Graphene-RGD Peptide Nanoisland Composites That Enhance the Osteogenesis of Human Adipose-Derived Mesenchymal Stem Cells

International Journal of Molecular Sciences
Ee-Seul KangTae-Hyung Kim

Abstract

Graphene derivatives have immense potential in stem cell research. Here, we report a three-dimensional graphene/arginine-glycine-aspartic acid (RGD) peptide nanoisland composite effective in guiding the osteogenesis of human adipose-derived mesenchymal stem cells (ADSCs). Amine-modified silica nanoparticles (SiNPs) were uniformly coated onto an indium tin oxide electrode (ITO), followed by graphene oxide (GO) encapsulation and electrochemical deposition of gold nanoparticles. A RGD-MAP-C peptide, with a triple-branched repeating RGD sequence and a terminal cysteine, was self-assembled onto the gold nanoparticles, generating the final three-dimensional graphene-RGD peptide nanoisland composite. We generated substrates with various gold nanoparticle-RGD peptide cluster densities, and found that the platform with the maximal number of clusters was most suitable for ADSC adhesion and spreading. Remarkably, the same platform was also highly efficient at guiding ADSC osteogenesis compared with other substrates, based on gene expression (alkaline phosphatase (ALP), runt-related transcription factor 2), enzyme activity (ALP), and calcium deposition. ADSCs induced to differentiate into osteoblasts showed higher calcium accumulations aft...Continue Reading

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Citations

Jul 28, 2018·International Journal of Molecular Sciences·Bogyu Choi, Soo-Hong Lee
May 23, 2018·Nanomaterials·Riccardo GuazzoBarbara Zavan
Nov 8, 2018·International Journal of Molecular Sciences·Chiara ArgentatiSabata Martino
Sep 16, 2020·Materials·Cheol-Hwi Kim, Tae-Hyung Kim
Jul 29, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yuhong QianZhuang Li

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