Three-Dimensional Histological Structures of the Human Dermis

Tissue Engineering. Part C, Methods
Yuzhen WangJun Wu

Abstract

Spatial information has been shown to be critical for cell differentiation and function. Therefore, a better understanding of skin microstructures is very important for biomimetic and bioengineered scaffolds of engineering skin. The purpose of the study was to generate collagen/elastin-based three-dimensional (3D) images of human dermis to further understand the microstructures of the skin, which is believed to be helpful in the fabrication of bionic engineered skin. Skin samples were fixed, embedded, serially sectioned, stained with aldehyde-fuchsin, and photographed as serial panoramas. Dermal subregions were divided according to dermal depth and distance to hair follicle. The porosity, pore diameters, and wall thickness of human acellular dermal matrix (ADM) were measured by microcomputed tomography (micro-CT). Three-dimensional reconstructed images of collagen and elastic fibers were generated. Our results showed that there were fewer elastic fibers in the subregions close to hair follicles than in the subregions far away from hair follicles (p<0.001), but the collagen fibers were evenly distributed. Both collagen and elastic fibers were found in fewer numbers in the layers either close to the epidermis or close to the hypo...Continue Reading

References

Jan 1, 1990·Archives of Dermatological Research·A B CuaH I Maibach
Jul 1, 1968·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S T ManderG G Carmichael
May 1, 1996·Burns : Journal of the International Society for Burn Injuries·Y TakamiR J Walter
May 15, 2002·Journal of Biomedical Materials Research·A K SalemK M Shakesheff
Jul 10, 2003·Journal of Shoulder and Elbow Surgery·Richard E DebskiPatrick J McMahon
Jul 26, 2003·Burns : Journal of the International Society for Burn Injuries·Paul P M van ZuijlenEsther Middelkoop
Nov 25, 2003·The Anatomical Record. Part B, the New Anatomist·Shao-Xiang ZhangYong-Ming Xie
Jul 28, 2004·Biomaterials·F J O'BrienL J Gibson
Nov 25, 2006·ORL; Journal for Oto-rhino-laryngology and Its Related Specialties·Haobing WangMads S Sorensen
May 24, 2007·Tissue Engineering·Richard D PriceHarshad A Navsaria
Jan 23, 2009·Journal of Biomechanics·Maurizio VentrePaolo A Netti
Sep 22, 2009·Journal of Biophotonics·Xiaoqin ZhuBifang Lin
Nov 18, 2010·Computer Methods in Biomechanics and Biomedical Engineering·Thomas S RauMartin Leinung
Mar 20, 2012·Annals of Biomedical Engineering·Aisling Ní AnnaidhGiuseppe Saccomandi
Jun 19, 2012·International Journal of Pharmaceutics·Gopinathan K MenonMajella E Lane
Jun 26, 2012·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Hanne JensenOddvar Myhre
Aug 14, 2012·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Pauline D VerhaegenPaul P van Zuijlen
Dec 25, 2012·Tissue Engineering. Part a·Shani ShiloOded Shoseyov
Jan 29, 2013·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Sadaf Hashim HussainTatyana R Humphreys
Jan 1, 2014·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·Aletta HazeveldYijin Ren
Jan 5, 2014·Acta Biomaterialia·Qin ZhangGuoping Chen
Apr 16, 2014·Journal of Nanoscience and Nanotechnology·Vuyiswa J Mkhabela, Suprakas Sinha Ray
Apr 23, 2014·Carbohydrate Polymers·Bijan Nasri-NasrabadiShahin Gavanji
Apr 26, 2014·International Journal of Polymeric Materials·Liang MaWei Li

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Citations

Jan 18, 2017·Journal of Tissue Engineering and Regenerative Medicine·Rui XuJun Wu
Dec 15, 2017·Journal of Microscopy·P LiuZ-C Hu
Sep 4, 2020·Cell and Tissue Banking·Lívia Contini MassiminoAna Maria de Guzzi Plepis
May 5, 2018·Archives of Dermatological Research·Norbert KissMárta Medvecz
Jul 1, 2020·Frontiers in Bioengineering and Biotechnology·Yuzhen WangJun Wu
Apr 14, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Zhiwen JianHesheng Xia
Feb 11, 2021·Biomechanics and Modeling in Mechanobiology·David SachsEdoardo Mazza
Aug 7, 2019·Colloids and Surfaces. B, Biointerfaces·Sónia P MiguelIlídio J Correia

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Methods Mentioned

BETA
scanning electron microscopy
X-ray
light microscopy

Software Mentioned

Adobe Photoshop
Amira
Adobe Photoshop CS5
MATLAB
DeclareMathSizes
SPSS
SCANCO

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