"Three-dimensional hybridization" with polyvalent DNA-gold nanoparticle conjugates.

Journal of the American Chemical Society
Sarah J HurstChad A Mirkin

Abstract

We have determined the minimum number of base pairings necessary to stabilize DNA-Au NP aggregates as a function of salt concentration for particles between 15 and 150 nm in diameter. Significantly, we find that sequences containing a single base pair interaction are capable of effecting hybridization between 150 nm DNA-Au NPs. While traditional DNA hybridization involves two strands interacting in one dimension (1D, Z), we propose that hybridization in the context of an aggregate of polyvalent DNA-Au NP conjugates occurs in three dimensions (many oligonucleotides oriented perpendicular to the X, Y plane engage in base pairing), making nanoparticle assembly possible with three or fewer base pairings per DNA strand. These studies enabled us to compare the stability of duplex DNA free in solution and bound to the nanoparticle surface. We estimate that 4-8, 6-19, or 8-33 additional DNA bases must be added to free duplex DNA to achieve melting temperatures equivalent to hybridized systems formed from 15, 60, or 150 nm DNA-Au NPs, respectively. In addition, we estimate that the equilibrium binding constant (K(eq)) for 15 nm DNA-Au NPs (3 base pairs) is approximately 3 orders of magnitude higher than the K(eq) for the corresponding n...Continue Reading

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Citations

Jun 2, 2012·ACS Applied Materials & Interfaces·Mohammed I ShukoorWeihong Tan
Nov 3, 2011·Journal of the American Chemical Society·Matthew R JonesChad A Mirkin
Jan 11, 2012·Journal of the American Chemical Society·Joshua I CutlerChad A Mirkin
May 9, 2012·Journal of the American Chemical Society·Zeljka KrpetićDuncan Graham
Apr 26, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Nishi BhattJuewen Liu
Aug 4, 2009·Nano Letters·Dan ZhengChad A Mirkin
Jan 24, 2013·Physical Chemistry Chemical Physics : PCCP·Lorenzo Di Michele, Erika Eiser
Apr 22, 2009·Nucleic Acids Research·Chunlai ChenXin Sheng Zhao
Feb 27, 2014·Nature Communications·Hyojin LeeJwa-Min Nam
Jun 7, 2011·The Journal of Chemical Physics·Terry J Schmitt, Thomas A Knotts
May 6, 2016·Journal of the American Chemical Society·Ryan V ThanerChad A Mirkin
May 19, 2016·ACS Nano·Benjamin D MyersVinayak P Dravid
Jul 2, 2016·Nano Letters·Silvia Hernández-AinsaUlrich F Keyser
May 8, 2009·Chembiochem : a European Journal of Chemical Biology·Martin MengClemens Richert
May 26, 2011·Chemical Society Reviews·Amit PatwaPhilippe Barthélémy
Jan 17, 2020·Angewandte Chemie·Yueyue ZhangXiaolei Zuo
Apr 24, 2013·Chemical Communications : Chem Comm·Alfonso LatorreÁlvaro Somoza
Apr 20, 2010·Angewandte Chemie·David A GiljohannChad A Mirkin
Jul 22, 2014·Angewandte Chemie·Matthew N O'BrienChad A Mirkin
Oct 21, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Bin LiuGang Wei
Jan 23, 2021·Journal of the American Chemical Society·Ho Fung ChengChad A Mirkin
Mar 31, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Bryan A Baker, Valeria T Milam
Jan 7, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Filip DelportJeroen Lammertyn
Jul 8, 2010·The Journal of Physical Chemistry Letters·One-Sun LeeGeorge C Schatz
Jul 2, 2014·Journal of the American Chemical Society·Resham J BangaChad A Mirkin
Nov 2, 2012·The Journal of Physical Chemistry. B·Jeehae ShinJuewen Liu
Jan 15, 2011·ACS Nano·Neeshma Dave, Juewen Liu
Sep 11, 2014·The Journal of Physical Chemistry. B·Taraknath MandalPrabal K Maiti
Nov 17, 2010·ACS Applied Materials & Interfaces·Ajfan BaeissaJuewen Liu

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