Three-dimensional image analysis of the mouse cochlea

Differentiation; Research in Biological Diversity
Graham D Wright, Henning F Horn

Abstract

The mouse has proven to be an essential model system for studying hearing loss. A key advantage of the mouse is the ability to image the sensory cells in the cochlea. Many different protocols exist for the dissection and imaging of the cochlea. Here we describe a method that utilizes confocal imaging of whole-mount preparations followed by 3D analysis using the Imaris software. The 3D analysis of confocal stacks has been successfully used for investigating a number of mouse tissues and developmental processes. We propose that this method is also a valuable tool to analyze the cellular and tissue organization of the sensory hair cells in the cochlea.

References

Jan 1, 1986·Hearing Research·H P Zenner
Sep 18, 2002·Progress in Neurobiology·Helene Benveniste, Steve Blackband
Apr 28, 2005·Hearing Research·Michel LeiboviciChristine Petit
May 20, 2006·Brain Research·Ning YuHong-Bo Zhao
Apr 7, 2007·The Journal of Membrane Biology·Takahisa AbeHideichi Shinkawa
Sep 25, 2007·The International Journal of Developmental Biology·Lilach M FriedmanKaren B Avraham
Nov 13, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sharon G Kujawa, M Charles Liberman
Nov 13, 2010·The Journal of Biological Chemistry·Kazuaki Homma, Peter Dallos
Dec 27, 2011·European Journal of Public Health·Gretchen StevensUNKNOWN Global Burden of Disease Hearing Loss Expert Group
Apr 26, 2012·Experimental Animals·Yoshiaki KikkawaHiromichi Yonekawa
Jan 26, 2013·The Journal of Clinical Investigation·Henning F HornKaren B Avraham
Nov 21, 2014·Nature Protocols·Alexander N CombesIan M Smyth
Feb 5, 2015·Journal of Visualized Experiments : JoVE·Khujista D HaqueChandrakala Puligilla

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Citations

May 1, 2021·Life·Kathrin MalfeldVerena Scheper

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