Three-dimensional image reconstruction of reconstituted smooth muscle thin filaments: effects of caldesmon

Biophysical Journal
J L HodgkinsonWilliam Lehman

Abstract

Caldesmon inhibits actomyosin ATPase and filament sliding in vitro, and therefore may play a role in modulating smooth and non-muscle motile activities. A bacterially expressed caldesmon fragment, 606C, which consists of the C-terminal 150 amino acids of the intact molecule, possesses the same inhibitory properties as full-length caldesmon and was used in our structural studies to examine caldesmon function. Three-dimensional image reconstruction was carried out from electron micrographs of negatively stained, reconstituted thin filaments consisting of actin and smooth muscle tropomyosin both with and without added 606C. Helically arranged actin monomers and tropomyosin strands were observed in both cases. In the absence of 606C, tropomyosin adopted a position on the inner edge of the outer domain of actin monomers, with an apparent connection to sub-domain 1 of actin. In 606C-containing filaments that inhibited acto-HMM ATPase activity, tropomyosin was found in a different position, in association with the inner domain of actin, away from the majority of strong myosin binding sites. The effect of caldesmon on tropomyosin position therefore differs from that of troponin on skeletal muscle filaments, implying that caldesmon and ...Continue Reading

Citations

May 26, 2004·Biochimica Et Biophysica Acta·Yurii S BorovikovRenata Dabrowska
Jun 19, 1998·Current Opinion in Structural Biology·A McGough
Mar 11, 2000·European Journal of Biochemistry·D I LevitskyN B Gusev
Oct 8, 1999·Molecular Biology of the Cell·D M HelfmanA D Bershadsky
Apr 9, 2008·Acta Crystallographica. Section D, Biological Crystallography·Michael R SawayaTodd O Yeates
Sep 22, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Elaine M SmolockRobert S Moreland
Feb 3, 2006·Journal of Biomedical Science·Jolanta KordowskaChih-Lueh Albert Wang
Nov 4, 2009·Journal of Molecular Biology·Xiaochuan Edward LiStefan Fischer
May 16, 2006·Journal of Molecular Biology·Kiran PantWilliam Lehman
May 9, 2006·Biochemical and Biophysical Research Communications·Natalia KulikovaYurii S Borovikov
Feb 2, 2000·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·W D EhringerF N Miller
Dec 2, 2008·Biochimica Et Biophysica Acta·Radosław SkórzewskiJoanna Moraczewska
Feb 16, 2011·Biophysical Journal·Xiaochuan Edward LiWilliam Lehman
Jul 19, 2002·Biophysical Journal·Andrea M ResetarJoseph M Chalovich
Dec 10, 2009·PloS One·Henry G ZotNguyen Van Minh
Sep 29, 2009·Biochemical and Biophysical Research Communications·Natalia KulikovaYurii S Borovikov
Apr 8, 2009·International Review of Cell and Molecular Biology·Jim Jung-Ching LinJian-Ping Jin
Feb 7, 2012·Journal of Molecular Biology·Srboljub M MijailovichMichael A Geeves
Jan 15, 1999·Acta Physiologica Scandinavica·J M ChalovichY D Chen
Jul 22, 2015·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Takeyuki Wakabayashi
Apr 4, 2009·Journal of Molecular Biology·William LehmanRoger Craig
Feb 17, 2005·Journal of Molecular Biology·Alnoor PiraniWilliam Lehman
Jan 30, 2004·American Journal of Physiology. Cell Physiology·Sita Somara, Khalil N Bitar
Oct 1, 2004·The Journal of Biological Chemistry·D Brian FosterC-L Albert Wang
Oct 27, 2004·The Journal of Biological Chemistry·Philip Graceffa, Andrew Mazurkie
Nov 15, 2007·Cell Motility and the Cytoskeleton·M J GreenbergJ R Moore
Jan 1, 2009·Journal of Muscle Research and Cell Motility·Kenneth C Holmes, William Lehman
Sep 24, 2005·Journal of Muscle Research and Cell Motility·Mechthild M Schroeter, Joseph M Chalovich
Apr 22, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Sita Somara, Khalil N Bitar
Feb 9, 2012·Journal of Muscle Research and Cell Motility·William Lehman, Kathleen G Morgan
Oct 16, 2007·The Journal of Biological Chemistry·Saira AnsariMohammed El-Mezgueldi
Nov 17, 2007·The Journal of Biological Chemistry·Robin MaytumMichael A Geeves

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