Three-dimensional imaging of the unsectioned adult spinal cord to assess axon regeneration and glial responses after injury

Nature Medicine
Ali ErtürkFrank Bradke


Studying regeneration in the central nervous system (CNS) is hampered by current histological and imaging techniques because they provide only partial information about axonal and glial reactions. Here we developed a tetrahydrofuran-based clearing procedure that renders fixed and unsectioned adult CNS tissue transparent and fully penetrable for optical imaging. In large spinal cord segments, we imaged fluorescently labeled cells by 'ultramicroscopy' and two-photon microscopy without the need for histological sectioning. We found that more than a year after injury growth-competent axons regenerated abundantly through the injury site. A few growth-incompetent axons could also regenerate when they bypassed the lesion. Moreover, we accurately determined quantitative changes of glial cells after spinal cord injury. Thus, clearing CNS tissue enables an unambiguous evaluation of axon regeneration and glial reactions. Our clearing procedure also renders other organs transparent, which makes this approach useful for a large number of preclinical paradigms.


Aug 5, 2000·The Journal of Steroid Biochemistry and Molecular Biology·F LabombardaA F De Nicola
Apr 12, 2002·Nature·Elizabeth J BradburyStephen B McMahon
May 31, 2002·Nature·Tadzia GrandPréStephen M Strittmatter
Mar 12, 2003·The Journal of Comparative Neurology·Oswald StewardMarc Tessier-Lavigne
Jan 22, 2004·Nature Reviews. Neuroscience·Jerry Silver, Jared H Miller
Mar 6, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jill R FaulknerMichael V Sofroniew
Apr 12, 2005·Nature Medicine·Martin KerschensteinerThomas Misgeld
May 11, 2005·Cell·Hui ZongLiqun Luo
Aug 24, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ali ErtürkFrank Bradke
Sep 20, 2008·Current Opinion in Neurobiology·Jeff W Lichtman, Joshua R Sanes
Nov 11, 2008·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Li WangXiao-Ming Xu
Apr 8, 2009·Current Opinion in Neurobiology·Edward M Callaway
Aug 12, 2010·PLoS Computational Biology·Hermann CuntzMichael Häusser

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