Three-Dimensional In Vitro Hydro- and Cryogel-Based Cell-Culture Models for the Study of Breast-Cancer Metastasis to Bone

Cancers
Laura J BrayCarsten Werner

Abstract

Bone is the most common site for breast-cancer invasion and metastasis, and it causes severe morbidity and mortality. A greater understanding of the mechanisms leading to bone-specific metastasis could improve therapeutic strategies and thus improve patient survival. While three-dimensional in vitro culture models provide valuable tools to investigate distinct heterocellular and environmental interactions, sophisticated organ-specific metastasis models are lacking. Previous models used to investigate breast-to-bone metastasis have relied on 2.5D or singular-scaffold methods, constraining the in situ mimicry of in vitro models. Glycosaminoglycan-based gels have demonstrated outstanding potential for tumor-engineering applications. Here, we developed advanced biphasic in vitro microenvironments that mimic breast-tumor tissue (MCF-7 and MDA-MB-231 in a hydrogel) spatially separated with a mineralized bone construct (human primary osteoblasts in a cryogel). These models allow distinct advantages over former models due to the ability to observe and manipulate cellular migration towards a bone construct. The gels allow for the binding of adhesion-mediating peptides and controlled release of signaling molecules. Moreover, mechanical a...Continue Reading

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Citations

Aug 14, 2019·Journal of Biomedical Materials Research. Part a·Mehdi RazaviAvnesh S Thakor
Nov 2, 2019·Biomaterials Science·Dejan HusmanCarsten Werner
Nov 7, 2019·JBMR Plus·Justin HamMichaela R Reagan
Oct 2, 2019·Frontiers in Bioengineering and Biotechnology·Laura J BrayNathalie Bock
May 3, 2020·Journal of Experimental & Clinical Cancer Research : CR·Somshuvra BhattacharyaPilar de la Puente
May 28, 2019·Integrative Biology : Quantitative Biosciences From Nano to Macro·Xueting MeiLidan You
Mar 29, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Majid SharifiMojtaba Falahati
Apr 3, 2020·Journal of Microbiology and Biotechnology·I Jyothilekshmi, N S Jayaprakash

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Methods Mentioned

BETA
Assay
atomic force microscopy
X-Ray

Software Mentioned

ImageJ
Volocity® 3D Image Analysis
Fiji

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