Three-dimensional islet graft histology: panoramic imaging of neural plasticity in sympathetic reinnervation of transplanted islets under the kidney capsule

American Journal of Physiology. Endocrinology and Metabolism
Jyuhn-Huarng JuangShiue-Cheng Tang

Abstract

Microscopic examination of transplanted islets in an ectopic environment provides information to evaluate islet engraftment, including revascularization and reinnervation. However, because of the dispersed nature of blood vessels and nerves, global visualization of the graft neurovascular network has been difficult. In this research we revealed the neurovascular network by preparing transparent mouse islet grafts under the kidney capsule with optical clearing to investigate the sympathetic reinnervation via three-dimensional confocal microscopy. Normoglycemic and streptozotocin-induced diabetic mice were used in syngeneic islet transplantation, with both groups maintaining euglycemia after transplantation. Triple staining of insulin/glucagon, blood vessels, and tyrosine hydroxylase (sympathetic marker) was used to reveal the graft microstructure, vasculature, and sympathetic innervation. Three weeks after transplantation, we observed perigraft sympathetic innervation similar to the peri-islet sympathetic innervation in the pancreas. Six weeks after transplantation, prominent intragraft, perivascular sympathetic innervation was achieved, resembling the pancreatic intraislet, perivascular sympathetic innervation in situ. Meanwhil...Continue Reading

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Citations

Sep 10, 2014·Diabetes, Obesity & Metabolism·S-C TangH-J Chien
Oct 27, 2015·International Journal of Obesity : Journal of the International Association for the Study of Obesity·H-J ChienS-C Tang
Dec 15, 2017·Journal of Microscopy·P LiuZ-C Hu
Jun 25, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Pei-Yu LinShiue-Cheng Tang
Sep 3, 2017·Diabetologia·Shiue-Cheng TangPankaj J Pasricha
Oct 18, 2018·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Bruna Andrade AguiarMaria Angelica Miglino
Aug 31, 2017·Diabetologia·Shiue-Cheng TangMichael S German
Sep 30, 2018·Current Diabetes Reports·Shiue-Cheng TangMartha Campbell-Thompson
Sep 9, 2017·Transplantation·Christian SchuetzUNKNOWN IPITA YIC Young Investigator Committee
Sep 12, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Hung-Jen ChienShiue-Cheng Tang
May 14, 2021·Frontiers in Endocrinology·Martha Campbell-Thompson, Shiue-Cheng Tang
Apr 27, 2021·American Journal of Physiology. Endocrinology and Metabolism·Chien-Chia ChenShiue-Cheng Tang
Jun 23, 2021·Trends in Endocrinology and Metabolism : TEM·Andreas Alvin Purnomo SoetedjoAdrian Kee Keong Teo

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