Three-dimensional mammalian tooth development using diceCT

Archives of Oral Biology
Qamariya NasrullahAlistair R Evans

Abstract

This study aims to develop the Diffusible Iodine-based Contrast-Enhanced CT (diceCT) method for non-destructive imaging of both soft and mineralised tissues. We sought to document the 3D spatio-temporal pattern of mammalian tooth development including multiple tooth classes and generations, using the tammar wallaby (Macropus eugenii) as a model species. We took microCT scans of developing fetuses and pouch young stained using Lugol's Iodine (I2KI) contrast agent. Stained versus unstained specimen comparisons were then made to investigate whether staining had improved visualisation of structures. Scan slices were compared to histological sections to confirm the identity of tissues and structures. Tissue layers were digitally segmented to create 3D models. DiceCT dramatically enhanced visual contrast of soft tissues, allowing differentiation between epithelial and mesenchymal layers. Subvolume scans at higher magnification achieved single-cell layer resolution within relatively large intact heads. We observed in-situ initiating teeth, which progressed through major stages of tooth development including morphogenesis and mineralisation. In addition, we traced the development of other mineralized and unmineralised tissues, such as ...Continue Reading

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Citations

Nov 30, 2020·Clinical Genetics·Ondrej BonczekBorivoj Vojtesek
Apr 4, 2021·Journal of Developmental Biology·Daisy Jihyung KoJulia C Boughner
Jun 27, 2021·Brain Structure & Function·Paul M GignacDolores Vazquez-Sanroman

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