Three-dimensional manipulation of single cells using surface acoustic waves

Proceedings of the National Academy of Sciences of the United States of America
Feng GuoTony Jun Huang

Abstract

The ability of surface acoustic waves to trap and manipulate micrometer-scale particles and biological cells has led to many applications involving "acoustic tweezers" in biology, chemistry, engineering, and medicine. Here, we present 3D acoustic tweezers, which use surface acoustic waves to create 3D trapping nodes for the capture and manipulation of microparticles and cells along three mutually orthogonal axes. In this method, we use standing-wave phase shifts to move particles or cells in-plane, whereas the amplitude of acoustic vibrations is used to control particle motion along an orthogonal plane. We demonstrate, through controlled experiments guided by simulations, how acoustic vibrations result in micromanipulations in a microfluidic chamber by invoking physical principles that underlie the formation and regulation of complex, volumetric trapping nodes of particles and biological cells. We further show how 3D acoustic tweezers can be used to pick up, translate, and print single cells and cell assemblies to create 2D and 3D structures in a precise, noninvasive, label-free, and contact-free manner.

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Citations

Jan 10, 2017·ACS Nano·Zhangming MaoTony Jun Huang
May 18, 2016·IEEE Transactions on Bio-medical Engineering·Yuta KurashinaJun Komotori
Mar 28, 2017·Advances in Colloid and Interface Science·Duyang ZangXingguo Geng
Dec 20, 2016·Lab on a Chip·Murat KaynakTony Jun Huang
Dec 28, 2016·Advanced Materials·Tailin XuShutao Wang
Nov 9, 2017·Lab on a Chip·ChunHui WuXing Cheng
Sep 5, 2017·Reports on Progress in Physics·Helen MillerMark C Leake
May 31, 2018·Soft Matter·Jasmine O CastroLeslie Y Yeo
Jun 27, 2018·Lab on a Chip·Armaghan FakhfouriAdrian Neild
Jul 3, 2018·Nanoscale·Shwathy RamesanLeslie Y Yeo
Sep 29, 2018·Nanotechnology·Yue WuFeng Guo
Feb 16, 2019·Lab on a Chip·Jinxin ZhangTony Jun Huang
Jan 22, 2019·Physical Biology·Xiaokan GuoJames Q Boedicker
Mar 29, 2019·Lab on a Chip·Bin ChenFeng Guo
Apr 11, 2019·Soft Matter·Minji KimJ Mark Meacham
Nov 28, 2019·Advanced Materials·Tanchen RenUtkan Demirci
Nov 30, 2019·Advanced Materials·Zhichao MaPeer Fischer
Apr 16, 2020·Lab on a Chip·Jinxin ZhangTony Jun Huang
Sep 27, 2019·Scientific Reports·Parth Chansoria, Rohan Shirwaiker
Jun 13, 2020·Annual Review of Analytical Chemistry·Peiran ZhangTony Jun Huang
May 22, 2020·Biofabrication·Hongwei CaiFeng Guo

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