Three dimensional microcarrier system in mesenchymal stem cell culture: a systematic review

Cell & Bioscience
Benson KohMuhammad Dain Yazid

Abstract

Stem cell-based regenerative medicine is a promising approach for tissue reconstruction. However, a large number of cells are needed in a typical clinical study, where conventional monolayer cultures might pose a limitation for scale-up. The purpose of this review was to systematically assess the application of microcarriers in Mesenchymal Stem Cell cultures. A comprehensive search was conducted in Medline via Ebscohost, Pubmed, and Scopus, and relevant studies published between 2015 and 2019 were selected. The literature search identified 53 related studies, but only 14 articles met the inclusion criteria. These include 7 utilised commercially available microcarriers, while the rest were formulated based on different surface characteristics, all of which are discussed in this review. Current applications of microcarriers were focused on MSC expansion and induction of MSCs into different lineages. These studies demonstrated that MSCs could proliferate in a microcarrier culture system in-fold compared to monolayer cultures, and the culture system could simulate a three-dimensional environment which induces cell differentiation. However, detailed studies are still required before this system were to be adapted into the scale of G...Continue Reading

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Citations

Dec 1, 2020·Frontiers in Pediatrics·Ling Ling LiauJia Xian Law
Feb 19, 2021·ACS Biomaterials Science & Engineering·Kuan Un WongGiselle C Yeo
Mar 19, 2021·Critical Reviews in Biotechnology·Alonso Ornelas-GonzálezMarco Rito-Palomares
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Oct 13, 2021·Science China. Life Sciences·Zi-Yuan FengHui-Qi Xie
Sep 29, 2021·Journal of Biomedical Materials Research. Part a·Anna Kornmuller, Lauren E Flynn

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Methods Mentioned

BETA
FCS
Scanning electron microscopy

Software Mentioned

Scopus
Ebscohost

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