Three-Dimensional Model of Dorsal Root Ganglion Explant as a Method of Studying Neurotrophic Factors in Regenerative Medicine

Biomedicines
Polina KlimovichEkaterina Semina

Abstract

Neurotrophiс factors play a key role in the development, differentiation, and survival of neurons and nerve regeneration. In the present study, we evaluated the effect of certain neurotrophic factors (NGF, BDNF, and GDNF) on axon growth and migration of Nestin-green fluorescent protein (GFP)-positive cells using a 3D model of dorsal root ganglion (DRG) explant culture in Matrigel. Our method generally represents a convenient model for assessing the effects of soluble factors and therapeutic agents on axon growth and nerve regeneration in R&D studies. By analyzing the DRG explants in ex vivo culture for 21 days, one can evaluate the parameters of neurite outgrowth and the rate of cell migration from the DRG explants into the Matrigel. For the current study, we used Nestin-GFP-expressing mice in which neural precursors express Nestin and the green fluorescent protein (GFP) under the same promoter. We revealed that GDNF significantly (two fold) stimulated axon outgrowth (p < 0.05), but not BDNF or NGF. It is well-known that axon growth can be stimulated by activated glial cells that fulfill a trophic function for regenerating nerves. For this reason, we evaluated the number of Nestin-GFP-positive cells that migrated from the DRG i...Continue Reading

References

Jul 1, 1976·Brain Research Bulletin·W J Freed
Jun 1, 1996·Neuroscience Research·C Ide
Jan 8, 1999·Microsurgery·S P FrostickG J Kemp
Aug 25, 2001·Annual Review of Neuroscience·E J Huang, L F Reichardt
Nov 27, 2001·Science·E CukiermanK M Yamada
Mar 12, 2002·The European Journal of Neuroscience·Eric G FinePatrick Aebischer
Jun 6, 2003·Progress in Neurobiology·Volkmar LessmannMarzia Malcangio
Jan 20, 2004·The Journal of Comparative Neurology·John L MignoneGrigori Enikolopov
Aug 3, 2005·Nature Reviews. Neuroscience·Bai LuNewton H Woo
Apr 9, 2008·Pharmacology & Therapeutics·Margaret I Davis
Feb 21, 2009·Neurosurgical Focus·Tessa Gordon
May 11, 2010·Journal of Communication Disorders·Tessa Gordon
May 23, 2012·Progress in Neurobiology·Ilary AllodiXavier Navarro
Nov 30, 2012·Journal of Translational Medicine·Luigi AloeLuigi Manni
Dec 6, 2012·Translational Neurodegeneration·April M Weissmiller, Chengbiao Wu
Aug 27, 2013·Methods in Molecular Biology·Jennifer GordonMartyn K White
Aug 3, 2014·Cold Spring Harbor Protocols·J Bradley Zuchero
Sep 16, 2014·Frontiers in Cellular Neuroscience·Maryna Baydyuk, Baoji Xu
Jun 3, 2015·Hand : Official Journal of the American Association for Hand Surgery·Danielle M WojtkiewiczSusan E Mackinnon
Aug 4, 2015·Frontiers in Molecular Neuroscience·Stefan A HoyngJoost Verhaagen
Sep 10, 2015·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·M KaragyaurV Tkachuk
Mar 15, 2016·Expert Review of Neurotherapeutics·Kristin Robin Ko, John P Frampton
Oct 18, 2016·Drug Discoveries & Therapeutics·Mehmet Emin ÖngerSüleyman Kaplan
May 19, 2017·SLAS Discovery·Ye Fang, Richard M Eglen
Jun 18, 2017·Cellular and Molecular Neurobiology·Przemysław KowiańskiJanusz Moryś
Sep 30, 2017·Frontiers in Cellular and Infection Microbiology·Grazieli MaboniSabine Tötemeyer
Oct 14, 2017·World Journal of Stem Cells·Sara Sayad Fathi, Arash Zaminy
Mar 9, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·M А BoldyrevaYe V Parfyonova

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
confocal microscopy
light microscopy

Software Mentioned

SigmaPlot11
Systat
Adobe
Photoshop
LAS AF
ImageJ

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.