Three-dimensional modelling of interchain sequence similarities and differences in the coiled-coil segments of keratin intermediate filament heterodimers highlight features important in assembly

Journal of Structural Biology
Thomasin A Smith, D A Parry

Abstract

Using structural data derived from crystal fragments of vimentin, three-dimensional models have been constructed for the major coiled-coil segments (1A, 1B and 2B) in epidermal and hair keratin intermediate filament molecules. Similarity and difference distributions arising from the heterodimer nature of the keratin molecules have been calculated, colour-coded for ease of observation and represented as movie clips. This approach has enabled the spatial distributions of the charged and apolar residues to be visualized along the seam between the chains and on the surface of the molecule, thus providing new insights into the features of the IF molecule that are important in assembly. An observation of note is that one face of both segment 1A and segment 1B is predominantly apolar and, furthermore, contains the bulk of the differences in the charged residues that occur between the two chains. The face rotated by 180 degrees contains far fewer apolar residues. This suggests the likely internal face of segments 1A and 1B and, hence, those sequence and spatial features that are important in assembly. In addition, the similarity distributions of the acidic and basic residues display a period of about 19 residues over much of each of th...Continue Reading

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Citations

Jul 10, 2009·The Journal of Investigative Dermatology·Lutz LangbeinJuergen Schweizer
May 26, 2010·Experimental Dermatology·Andrea TrostKamil Onder
Oct 24, 2013·Journal of Structural Biology·David A D Parry
Aug 4, 2015·Physical Chemistry Chemical Physics : PCCP·Patrick DuchsteinDirk Zahn
Apr 25, 2018·Scientific Reports·Annika ThomerJulia Metzger
Jul 15, 2015·Journal of Medical Genetics·Muhammad AnsarSuzanne M Leal
Sep 12, 2012·Biomacromolecules·Chia-Ching Chou, Markus J Buehler
Feb 2, 2018·The Journal of Physical Chemistry. B·Kenkoh S EndohFlorian Müller-Plathe

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