Three-dimensional models and structure analysis of corynemycolyltransferases in Corynebacterium glutamicum and Corynebacterium efficiens

International Journal of Biological Macromolecules
Swathi AdindlaLalitha Guruprasad

Abstract

The corynemycolyltransferase proteins were identified from Corynebacterium glutamicum and Corynebacterium efficiens genomes using computational tools available in the public domain. Three-dimensional models were constructed for corynemycolyltransferases based on the crystal structures of related mycolyltransferases in Mycobacterium tuberculosis using the comparative modeling methods. The corynemycolyltransferases share overall an alpha/beta-fold characteristic of the mycolyltransferases despite low sequence identity (<20%) shared by some of the corynemycolyltransferases. However, a significant difference is observed in the region between amino acid residues Trp82-Trp97 and Ala222-Asn223 corresponding to mycolyltransferases. The specificity pockets defined by interactions with the trehalose substrate observed in the crystal structure complex of Ag85B mycolyltransferase (PDB code: 1F0P) suggests that trehalose may not bind some corynemycolyltransferases. This is due to critical mutations in corynemycolyltransferase binding subsites that lead to loss of equivalent side-chain interactions with trehalose and unfavorable steric interactions, particularly, in the case of cmytC gene and the protein corresponding to the gene identifier ...Continue Reading

References

Jan 18, 1976·Biochimica Et Biophysica Acta·M A Lanéelle, J Asselineau
Dec 1, 1992·Microbiological Reviews·H G Wiker, M Harboe
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1968·Advances in Protein Chemistry·G N Ramachandran, V Sasisekharan
Jan 1, 1995·Annual Review of Biochemistry·P J Brennan, H Nikaido
Dec 5, 1993·Journal of Molecular Biology·A Sali, T L Blundell
Aug 22, 1997·Science·J S SchoreyE J Brown
Feb 28, 1998·The Journal of Biological Chemistry·M NaitoT Yamada
Nov 26, 1998·Progress in Lipid Research·C E BarryY Yuan
Mar 10, 2001·Nature·S T ColeB G Barrell
Apr 10, 2002·Letters in Applied Microbiology·L KremerG S Besra

❮ Previous
Next ❯

Related Concepts

Related Feeds