Three-dimensional molecular mapping in a microfluidic mixing device using fluorescence lifetime imaging

Optics Letters
Tom RobinsonPaul M W French

Abstract

Fluorescence lifetime imaging (FLIM) is used to quantitatively map the concentration of a small molecule in three dimensions in a microfluidic mixing device. The resulting experimental data are compared with computational fluid-dynamics (CFD) simulations. A line-scanning semiconfocal FLIM microscope allows the full mixing profile to be imaged in a single scan with submicrometer resolution over an arbitrary channel length from the point of confluence. Following experimental and CFD optimization, mixing times down to 1.3+/-0.4 ms were achieved with the single-layer microfluidic device.

References

Dec 27, 2005·The Journal of Chemical Physics·Glen I RedfordRobert M Clegg
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Mar 17, 2007·Microscopy Research and Technique·Pieter De BeulePaul M W French

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Citations

Mar 10, 2009·Current Opinion in Biotechnology·James A LevittKlaus Suhling
Apr 4, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hellen C Ishikawa-AnkerholdGregor P C Drummen
Aug 20, 2010·Lab on a Chip·Ye TianHong-Bo Sun
Jun 2, 2016·Reports on Progress in Physics·Thomas Andrew Waigh
Apr 30, 2011·Lab on a Chip·YongTae KimWilliam C Messner
Dec 24, 2020·Biosensors·Yung-Tsan ChenJian-Jang Huang
Aug 6, 2010·Analytical Chemistry·Fabrice GielenJoshua B Edel

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