Three dimensional passivated-electrode insulator-based dielectrophoresis

Biomicrofluidics
Diana NakiddeMasoud Agah

Abstract

In this study, a 3D passivated-electrode, insulator-based dielectrophoresis microchip (3D πDEP) is presented. This technology combines the benefits of electrode-based DEP, insulator-based DEP, and three dimensional insulating features with the goal of improving trapping efficiency of biological species at low applied signals and fostering wide frequency range operation of the microfluidic device. The 3D πDEP chips were fabricated by making 3D structures in silicon using reactive ion etching. The reusable electrodes are deposited on second glass substrate and then aligned to the microfluidic channel to capacitively couple the electric signal through a 100 μm glass slide. The 3D insulating structures generate high electric field gradients, which ultimately increases the DEP force. To demonstrate the capabilities of 3D πDEP, Staphylococcus aureus was trapped from water samples under varied electrical environments. Trapping efficiencies of 100% were obtained at flow rates as high as 350 μl/h and 70% at flow rates as high as 750 μl/h. Additionally, for live bacteria samples, 100% trapping was demonstrated over a wide frequency range from 50 to 400 kHz with an amplitude applied signal of 200 Vpp. 20% trapping of bacteria was observed...Continue Reading

References

Nov 1, 1996·Journal of Biotechnology·G H MarkxR Pethig
Aug 1, 1997·Biophysical Journal·Y HuangP R Gascoyne
May 24, 2006·Biomedical Microdevices·T P Hunt, R M Westervelt
May 2, 2007·Bioelectromagnetics·A SanchisJ M Miranda
Jul 13, 2007·Analytical and Bioanalytical Chemistry·Rafael V DavalosBlake A Simmons
May 20, 2008·Biomedical Microdevices·Poorya SabounchiRafael V Davalos
Feb 5, 2009·Analytical and Bioanalytical Chemistry·José I Martínez-LópezBlanca H Lapizco-Encinas
Feb 4, 2010·Lab on a Chip·Hadi ShafieeRafael V Davalos
Jul 28, 2011·Electrophoresis·Sriram SridharanXiangchun Xuan
Feb 9, 2012·Lab on a Chip·William A BraffCullen R Buie
Aug 18, 2012·Electrophoresis·Phillip Zellner, Masoud Agah
Jul 3, 2013·Analytical and Bioanalytical Chemistry·Phillip ZellnerMasoud Agah
Oct 29, 2013·Analytical and Bioanalytical Chemistry·Tyler ShakeMasoud Agah
Jan 10, 2014·Biomicrofluidics·Mohammed AlshareefGuiren Wang
Jun 25, 2014·Biomicrofluidics·Jinghui LuoAlexandra Ros

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Citations

Dec 18, 2015·Biomicrofluidics·Alexandra LaLondeBlanca H Lapizco-Encinas
Aug 17, 2018·Electrophoresis·Blanca H Lapizco-Encinas
Jan 29, 2020·Journal of Separation Science·Kruthika KikkeriMasoud Agah
Dec 7, 2019·Electrophoresis·Junyan ZhangYongxin Song
Nov 10, 2020·Electrophoresis·Cinthia J Ramirez-MurilloVictor H Perez-Gonzalez
Nov 1, 2018·The Science of the Total Environment·Chao QinKang Xia

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