Three-dimensional poly-(ε-caprolactone) nanofibrous scaffolds directly promote the cardiomyocyte differentiation of murine-induced pluripotent stem cells through Wnt/β-catenin signaling

BMC Cell Biology
Yan ChenQiang-Sun Zheng

Abstract

Environmental factors are important for stem cell lineage specification, and increasing evidence indicates that the nanoscale geometry/topography of the extracellular matrix (ECM) directs stem cell fate. Recently, many three-dimensional (3D) biomimetic nanofibrous scaffolds resembling many characteristics of the native ECM have been used in stem cell-based myocardial tissue engineering. However, the biophysical role and underlying mechanism of 3D nanofibrous scaffolds in cardiomyocyte differentiation of induced pluripotent stem cells (iPSCs) remain unclear. Here, we fabricated a 3D poly-(ε-caprolactone) (PCL) nanofibrous scaffold using the electrospinning method and verified its nanotopography and porous structure by scanning electron microscopy. We seeded murine iPSCs (miPSCs) directly on the 3D PCL nanofibrous scaffold and initiated non-directed, spontaneous differentiation using the monolayer method. After the 3D PCL nanofibrous scaffold was gelatin coated, it was suitable for monolayer miPSC cultivation and cardiomyocyte differentiation. At day 15 of differentiation, miPSCs differentiated into functional cardiomyocytes on the 3D PCL nanofibrous scaffold as evidenced by positive immunostaining of cardiac-specific proteins in...Continue Reading

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Apr 14, 2016·Nanomedicine·Manus BiggsDimitrios I Zeugolis
Nov 9, 2016·Acta Biomaterialia·Maria KitsaraPhilippe Menasché
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Dec 2, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Divya SridharanMahmood Khan
Aug 14, 2019·International Journal of Biological Macromolecules·Esra TürkerAhu Arslan Yildiz

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Methods Mentioned

BETA
PCR
PCL
electrophoresis
protein assay
FACS

Software Mentioned

CellQuest
ImageJ

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