Three-dimensional printed multiphasic scaffolds with stratified cell-laden gelatin methacrylate hydrogels for biomimetic tendon-to-bone interface engineering

Journal of Orthopaedic Translation
Yi CaoQingfeng Li

Abstract

The anatomical properties of the enthesis of the rotator cuff are hardly regained during the process of healing. The tendon-to-bone interface is normally replaced by fibrovascular tissue instead of interposition fibrocartilage, which impairs biomechanics in the shoulder and causes dysfunction. Tissue engineering offers a promising strategy to regenerate a biomimetic interface. Here, we report heterogeneous tendon-to-bone interface engineering based on a 3D-printed multiphasic scaffold. A multiphasic poly(ε-caprolactone) (PCL)-PCL/tricalcium phosphate (TCP)-PCL/TCP porous scaffold was manufactured using 3D printing technology. The three phases of the scaffold were designed to mimic the graded tissue regions in the tendon-to-bone interface-tendon, fibrocartilage, and bone. Fibroblasts, bone marrow-derived mesenchymal stem cells, and osteoblasts were separately encapsulated in gelatin methacrylate (GelMA) and loaded seriatim on the relevant phases of the scaffold, by which a cells/GelMA-multiphasic scaffold (C/G-MS) construct, replicating the native interface, was fabricated. Cell proliferation, viability, and chondrogenic differentiation were evaluated in vitro. The C/G-MS constructs were further examined to determine the potenti...Continue Reading

Citations

Nov 13, 2020·Tissue Engineering. Part B, Reviews·Wendy BalestriYvonne Reinwald
Mar 6, 2021·Bioactive Materials·Tingyun LeiZi Yin
Oct 2, 2021·Advanced Materials·Hossein RavanbakhshYu Shrike Zhang

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Methods Mentioned

BETA
delamination
PCL
scanning electron microscopy
light microscopy

Software Mentioned

Perfactory Software Suite
EnvisionTEC
SPSS
MicroCT

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