Three-dimensional printing and in vitro evaluation of poly(3-hydroxybutyrate) scaffolds functionalized with osteogenic growth peptide for tissue engineering

Materials Science & Engineering. C, Materials for Biological Applications
Sybele SaskaJoni Augusto Cirelli

Abstract

Poly(3-hydroxybutyrate) (PHB) is a biodegradable and thermoprocessable biopolymer, making it a promising candidate for applications in tissue engineering. In the present study a structural characterization and in vitro evaluation were performed on PHB scaffolds produced by additive manufacturing via selective laser sintering (SLS), followed by post-printing functionalization with osteogenic growth peptide (OGP) and its C-terminal sequence OGP(10-14). The PHB scaffolds were characterized, including their morphology, porosity, thermal and mechanical properties, moreover OGP release. The results showed that SLS technology allowed the sintering of the PHB scaffolds with a hierarchical structure with interconnected pores and intrinsic porosity (porosity of 55.8 ± 0.7% and pore size in the 500-700 μm range), and good mechanical properties. Furthermore, the SLS technology did not change thermal properties of PHB polymer. The OGP release profile showed that PHB scaffold promoted a controlled release above 72 h. In vitro assays using rat bone marrow stem cells showed good cell viability/proliferation in all the PHB scaffolds. Additionally, SEM images suggested advanced morphological differentiation in the groups containing osteogenic gr...Continue Reading

Citations

May 20, 2020·Advanced Healthcare Materials·Fan Zhang, Martin W King
Mar 25, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Sven H DiermannHan Huang
Mar 10, 2020·Journal of Healthcare Engineering·Massimo MartorelliSverio Maietta
Feb 21, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·D BichoJ M Oliveira
Sep 12, 2020·Pharmaceutics·Tania LimongiEnzo di Fabrizio
Apr 14, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Jay M Korde, Balasubramanian Kandasubramanian
Feb 25, 2020·Journal of the Mechanical Behavior of Biomedical Materials·Raffaella PecciCostantino Del Gaudio
Feb 11, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Moushmi GoswamiSeeram Ramakrishna
May 17, 2021·International Journal of Biological Macromolecules· AnjanaSaswata Goswami
Jul 13, 2021·Frontiers in Bioengineering and Biotechnology·Aurelio Salerno, Paolo A Netti

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