Three-Dimensional Printing of Vitrification Loop Prototypes for Aquatic Species

Zebrafish
Nolan J TierschTerrence R Tiersch

Abstract

Vitrification is a method of cryopreservation that freezes samples rapidly, while forming an amorphous solid ("glass"), typically in small (μL) volumes. The goal of this project was to create, by three-dimensional (3D) printing, open vitrification devices based on an elliptical loop that could be efficiently used and stored. Vitrification efforts can benefit from the application of 3D printing, and to begin integration of this technology, we addressed four main variables: thermoplastic filament type, loop length, loop height, and method of loading. Our objectives were to: (1) design vitrification loops with varied dimensions; (2) print prototype loops for testing; (3) evaluate loading methods for the devices; and (4) classify vitrification responses to multiple device configurations. The various configurations were designed digitally using 3D CAD (Computer Aided Design) software, and prototype devices were produced with MakerBot® 3D printers. The thermoplastic filaments used to produce devices were acrylonitrile butadiene styrene (ABS) and polylactic acid (PLA). Vitrification devices were characterized by the film volumes formed with different methods of loading (pipetting or submersion). Frozen films were classified to determi...Continue Reading

References

Aug 24, 2002·Biology of Reproduction·M HagedornB S Pukazhenthi
Sep 24, 2005·Reproductive Biomedicine Online·Masashige KuwayamaStanley P Leibo
Apr 20, 2006·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·P J Brennan
Feb 16, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Shogo HigakiYoshiyuki Takahashi
Sep 8, 2011·Cryobiology·Rafael Cuevas-UribeTerrence R Tiersch
Feb 12, 2014·Cryobiology·Leandro Cesar GodoyTiantian Zhang
Feb 6, 2016·PloS One·Francisco Marco-JiménezJosé Salvador Vicente
Dec 16, 2017·North American Journal of Aquaculture·Nolan J Tiersch, Terrence R Tiersch

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Citations

Nov 19, 2019·North American Journal of Aquaculture·William M ChildressTerrence R Tiersch
Aug 25, 2020·Aquacultural Engineering·Connor J TierschWilliam T Monroe

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Software Mentioned

Proc
MakerBot Desktop
SAS
Autodesk Inventor
Proc Glimmix

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