Three-dimensional reconstruction by cryoelectron microscopy of the giant hemoglobin of the polychaete worm Alvinella pompejana

Journal of Molecular Biology
F de HaasJ N Lamy

Abstract

A frozen-hydrated specimen of the hexagonal bilayer hemoglobin (HBL Hb) from the deep-sea hydrothermal vent polychaete worm Alvinella pompejana, the most thermophilic metazoan known to date, was observed in the electron microscope and subjected to three-dimensional (3D) reconstruction by the method of random conical tilt series. At a resolution of 34.6 A by the differential phase residual method and 27.7 A by the Fourier shell correlation method, the 3D volume possesses a D6 point-group symmetry. While in previous 3D reconstructions of annelid and vestimentiferan Hbs the vertices of the upper layer were 16 degrees rotated compared with those of the lower layer, in Alvinella Hb the vertices of the two hexagonal layers are almost perfectly eclipsed when viewed along the 6-fold axis. As in the HBL Hbs of Riftia pachyptila and Macrobdella decora, a central linker complex is decorated by 12 hollow globular substructures (HGS). The linker complex comprises (1) a central hexagonal toroid, (2) two internal bracelets onto which the HGSs are built, and (3) six connections between the two hexagonal layers. Each HGS is composed of six masses, which are separated when the volume is displayed at high threshold, plus one additional mass invol...Continue Reading

Citations

Apr 11, 2001·The Journal of Biological Chemistry·A PallaviciniG Lanfranchi
Mar 29, 2001·Physiological Reviews·R E Weber, S N Vinogradov
Jun 10, 2015·Acta Crystallographica. Section D, Biological Crystallography·José Fernando Ruggiero BachegaEduardo Horjales Reboredo
Jan 10, 2018·Bioengineering & Translational Medicine·Devon ZimmermanJacob J Elmer
Feb 17, 2001·Biochemical and Biophysical Research Communications·S DewildeL Moens
Feb 13, 2001·Micron : the International Research and Review Journal for Microscopy·J R HarrisM Adrian
Mar 24, 2004·Micron : the International Research and Review Journal for Microscopy·Serge N Vinogradov

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