Three-dimensional reconstruction from reduced sets of very noisy images acquired following a single-axis tilt schema: application of a new three-dimensional reconstruction algorithm and objective comparison with weighted backprojection

Journal of Structural Biology
R MarabiniJ M Carazo

Abstract

In this work we propose a reconstruction algorithm (ART with blobs) that has not been previously used in electron Tomography and we compare it with the standard method in the field (weighted back projection, WBP). We assume that only a limited set of very noisy images, collected around a single axis tilt, is available; which is a typical situation in Electron Tomography. In general, the reconstruction problem is underdetermined (due to the limited number of projections) and the data are inconsistent (due to the high level of noise). The evaluation of the results is performed in a rigorous way by a task-oriented approach which makes use of numerical observers. ART with blobs outperforms WBP for a number of key tasks. Results are presented both for simplified line integral data and for realistic simulations of macromolecular structures embedded in amorphous ice.

References

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Citations

Jul 6, 2007·European Biophysics Journal : EBJ·C O S SorzanoS Marco
Aug 6, 2002·Journal of Structural Biology·José-Jesús FernándezJosé-María Carazo
Dec 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Eric Todd QuintoOzan Oktem
Jun 15, 2005·Annual Review of Biochemistry·Vladan LucićWolfgang Baumeister
Feb 12, 2014·PloS One·Garrett KatzPaul Gottlieb
Jun 5, 2012·Micron : the International Research and Review Journal for Microscopy·Jose-Jesus Fernandez
Jul 8, 2015·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·Masih NilchianMichael Unser
Dec 21, 2007·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·Junghoon LeeJohn E Johnson
Oct 13, 2004·Journal of Structural Biology·C O S SorzanoA Pascual-Montano
May 23, 2002·IEEE Transactions on Medical Imaging·Jayaram K Udupa, Gabor T Herman
Sep 21, 2004·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·Roberto MarabiniGabor T Herman
Dec 12, 2012·Virology·Ricardo C Guerrero-Ferreira, Elizabeth R Wright
Jul 27, 2001·Journal of Structural Biology·C O SorzanoJ M Carazo
Mar 10, 2004·Physics in Medicine and Biology·C O S SorzanoJ M Carazo
Jan 10, 2018·BioMed Research International·C O S SorzanoJ M Carazo
Jun 3, 2011·Journal of Microscopy·H RullgårdO Oktem

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