PMID: 6537991Feb 9, 1984Paper

Three-dimensional reconstruction from tilted sections of fish muscle M-band

Nature
P K Luther, R A Crowther

Abstract

Fish muscle provides a particularly suitable specimen for studying the organization of thick filaments in vertebrate striated muscle because the filaments are arranged with a single orientation on a well ordered hexagonal lattice. The M-band consists of sets of cross-links, which join the myosin filaments in the middle of the A-band. Previous work concluded that the fish muscle M-band had the local symmetry of the dihedral point group 32. We present here a quantitative analysis of transverse sections of the M-band, using general methods developed for crystalline layers, to combine various tilted views. The three-dimensional map computed to a resolution of about 70 A confirms previous findings; it shows new features of the thick filament structure in the M-band region and provides new information on the use of three-dimensional reconstruction from sectioned biological material. The accompanying paper describes a novel technique of three-dimensional reconstruction of the fish M-band from a single view of a slightly oblique plastic section.

Citations

Sep 1, 1996·Journal of Morphology·P K LutherP L Forey
Jul 1, 1995·Microscopy Research and Technique·J van MarleH van Veen
Jul 9, 1999·Reviews of Physiology, Biochemistry and Pharmacology·D O FürstP F van der Ven
Oct 1, 1991·Ultramicroscopy·K A Taylor, R A Crowther
Jul 1, 1988·Journal of Ultrastructure and Molecular Structure Research·A C EdmanM Sjöström
May 1, 1989·The Journal of Cell Biology·N Q Cheng, J F Deatherage
Jun 1, 1991·The Journal of Cell Biology·P K Luther
Sep 13, 2015·Journal of Molecular Biology·Thomas BurgoynePradeep K Luther
Dec 2, 2004·Journal of Molecular Biology·Stephan LangeElisabeth Ehler
Feb 14, 2006·Journal of Muscle Research and Cell Motility·Stephan LangeElisabeth Ehler
Jul 20, 2019·Proceedings of the National Academy of Sciences of the United States of America·Thomas BurgoynePradeep K Luther
Dec 1, 1985·Journal of Muscle Research and Cell Motility·M BählerH M Eppenberger
Dec 1, 1994·Journal of Muscle Research and Cell Motility·H T PaskJ M Squire
Nov 16, 1992·Physical Review Letters·H DelacroixP Mariani

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.