Three-dimensional reconstruction of chromatin fibers

Journal of Biomolecular Structure & Dynamics
J A SubiranaJ Frank

Abstract

We have obtained tilt series of thin sections of chromatin fibers embedded in araldite-epon and stained with uranyl acetate. The tilt series contain between 12 and 18 micrographs, spanning tilt ranges between 84 and 117 degrees. Reconstructions in three dimensions have been obtained by filtered back-projection from each tilt series. The reconstructions have been low-pass filtered in order to reduce the amount of noise. In the reconstructions it is possible to approximately localize the nucleosomes. In several regions they show a clear zigzag arrangement, but in other regions it is difficult to determine the sequence of the nucleosomes. In any case there is no clear indication of a solenoidal arrangement. We discuss the rules which may give rise to a 3-D arrangement of the nucleosomal zigzag.

References

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Citations

Jan 1, 1985·Chromosoma·J A SubiranaJ Frank
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·B F McEwenJ Frank
May 20, 1999·Journal of Biomolecular Structure & Dynamics·W A Krajewski
Oct 12, 1987·Nucleic Acids Research·R D DrinkwaterL A Burgoyne
May 18, 2005·Biochimica Et Biophysica Acta·Brad J Marsh
Aug 1, 1988·Journal of Electron Microscopy Technique·M Radermacher
Apr 14, 1995·The Journal of Biological Chemistry·K van Holde, J Zlatanova
Feb 25, 1985·FEBS Letters·V MakarovI Pashev
May 11, 1992·FEBS Letters·J A Subirana

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