Three-dimensional reconstruction of internal fascicles and microvascular structures of human peripheral nerves

International Journal for Numerical Methods in Biomedical Engineering
Li-Wei YanLiqiang Gu

Abstract

Biofabricated nanostructured and microstructured scaffolds have exhibited great potential for nerve tissue regeneration and functional restoration, and prevascularization and biotransportation within 3D fascicle structures are critical. Unfortunately, an ideal internal fascicle and microvascular model of human peripheral nerves is lacking. In this study, we used microcomputed tomography (microCT) to acquire high-resolution images of the human sciatic nerve. We propose a novel deep-learning network technique, called ResNetH3D-Unet, to segment fascicles and microvascular structures. We reconstructed 3D intraneural fascicles and microvascular topography to quantify the fascicle volume ratio (FVR), microvascular volume ratio (MVR), microvascular to fascicle volume ratio (MFVR), fascicle surface area to volume ratio (FSAVR), and microvascular surface area to volume ratio (MSAVR) of human samples. The frequency distributions of the fascicle diameter, microvascular diameter, and fascicle-to-microvasculature distance were analyzed. The obtained microCT analysis and reconstruction provided high-resolution microstructures of human peripheral nerves. Our proposed ResNetH3D-Unet method for fascicle and microvasculature segmentation yielded...Continue Reading

References

Jul 1, 1997·Journal of Anatomy·D W Zochodne, C Nguyen
Oct 7, 2003·Annual Review of Biomedical Engineering·Christine E Schmidt, Jennie Baier Leach
Oct 21, 2003·Journal of Biomedical Materials Research. Part a·Satoru YoshiiMasao Akagi
May 6, 2005·Brain : a Journal of Neurology·Xiaodong WangXiaosong Gu
Sep 3, 2005·Biomaterials·Michael J MooreMichael J Yaszemski
Jul 24, 2007·Journal of Neuroscience Methods·Jianming Li, Riyi Shi
Sep 8, 2010·Bone·Michael DoubeSandra J Shefelbine
Apr 3, 2012·Acta Biomaterialia·Qiang ZhangDavid L Kaplan
Jan 3, 2013·Journal of the Peripheral Nervous System : JPNS·Mair ZamirJ Kevin Shoemaker
Aug 3, 2013·Reviews in the Neurosciences·Xupeng GaoJiang Peng
Oct 22, 2014·Medical Image Computing and Computer-assisted Intervention : MICCAI·Holger R RothRonald M Summers
Oct 27, 2014·Nature Materials·Ulrike G K WegstRobert O Ritchie
Nov 26, 2015·Scientific Reports·Yingchun ZhongCory J Xian
Mar 1, 2016·Advanced Functional Materials·Blake N JohnsonMichael C McAlpine
Oct 13, 2016·Journal of Digital Imaging·Alvin RajkomarJohn Mongan
Jan 20, 2018·Advanced Healthcare Materials·Paul A WieringaLorenzo Moroni
Mar 24, 2018·Biofabrication·Eugen B PetcuPaul D Dalton
Jun 16, 2018·Computer Methods and Programs in Biomedicine·Feiwei QinArtur Grudtsin
Apr 10, 2019·Polymers·Sanjairaj VijayavenkataramanJerry Ying Hsi Fuh

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.