Three-dimensional reconstruction of the mammalian centriole from cryoelectron micrographs: the use of common lines for orientation and alignment

Journal of Structural Biology
J KenneyS D Fuller

Abstract

The microtubule organizing center of the animal cell (S. D. Fuller et al., 1992, Curr. Opin. Struct. Biol. 2, 264-274; D. M. Glover et al., 1993, Sci. Am. 268, 62-68; E. B. Wilson, 1925), (The Cell in Development and Heredity) comprises two centrioles and the pericentriolar material. We have completed several three-dimensional reconstructions of individual centrioles from tilt series of cryoelectron micrographs. The reconstruction procedure uses minimization of the common lines residual to define the orientation of the centriolar minefold symmetry axis and then uses this symmetry to generate a structure by weighted backprojection to 28-nm resolution. Many of the features of these reconstructions agree with previous, conventional transmission electron microscopy studies (M. Paintrand et al., 1992, J. Struct. Biol. 108, 107-128). The microtubule barrel of the centriole is roughly 500 nm long and 300 nm in diameter and the microtubule bundles appear to taper toward the distal end. In addition, we see a handedness to the pericentriolar material at the base (distal end) of the centriole which is opposite to the skew of the microtubule triplets. The region at which the microtubule barrel joins this base is intriguingly complex and in...Continue Reading

References

Mar 1, 1992·Journal of Structural Biology·M PaintrandM Bornens
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·F TournierM Bornens
May 1, 1990·The Journal of Cell Biology·R S Kochanski, G G Borisy
Aug 1, 1988·Journal of Electron Microscopy Technique·M Radermacher
Jan 1, 1987·Cell Motility and the Cytoskeleton·M BornensE Karsenti
May 27, 1971·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R A Crowther
Aug 1, 1972·The Journal of Cell Biology·R G Anderson
Jun 1, 1993·Scientific American·D M GloverJ W Raff
Jan 1, 1996·Journal of Structural Biology·S D FullerT S Baker
Jan 1, 1996·Journal of Structural Biology·B SheehanM Yeager
Dec 1, 1995·Current Biology : CB·S D FullerE Karsenti
Sep 15, 1996·Structure·D ChrétienR H Wade
Sep 1, 1996·Trends in Cell Biology·B M Lange, K Gull

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Citations

Jul 16, 2008·Biochemistry. Biokhimii︠a︡·I B Alieva, R E Uzbekov
Nov 29, 2015·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Lisa I Mullee, Ciaran G Morrison
Oct 8, 2008·Microscopy Research and Technique·Rana IbrahimSergio Marco
Feb 7, 1998·Journal of Structural Biology·D ChrétienE Karsenti
Jul 15, 2009·Seminars in Cell & Developmental Biology·Sigrid Hoyer-Fender
May 13, 2003·Cell Motility and the Cytoskeleton·Young Y OuJerome B Rattner
Aug 19, 2007·Journal of Cellular Physiology·Jeffrey L Salisbury

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