Three-dimensional reconstruction of the distribution of elemental tags in single cells using laser ablation ICP-mass spectrometry via registration approaches

Analytical and Bioanalytical Chemistry
Stijn J M Van MalderenFrank Vanhaecke

Abstract

This paper describes a workflow towards the reconstruction of the three-dimensional elemental distribution profile within human cervical carcinoma cells (HeLa), at a spatial resolution down to 1 μm, employing state-of-the-art laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) instrumentation. The suspended cells underwent a series of fixation/embedding protocols and were stained with uranyl acetate and an Ir-based DNA intercalator. A priori, laboratory-based absorption micro-computed tomography (μ-CT) was applied to acquire a reference frame of the morphology of the cells and their spatial distribution before sectioning. After CT analysis, a trimmed 300 × 300 × 300 μm3 block was sectioned into a sequential series of 132 sections with a thickness of 2 μm, which were subjected to LA-ICP-MS imaging. A pixel acquisition rate of 250 pixels s-1 was achieved, through a bidirectional scanning strategy. After acquisition, the two-dimensional elemental images were reconstructed using the timestamps in the laser log file. The synchronization of the data required an improved optimization algorithm, which forces the pixels of scans in different ablation directions to be spatially coherent in the direction orthogonal to ...Continue Reading

References

Sep 12, 2000·Nature Cell Biology·K MonierK F Sullivan
Aug 9, 2003·IEEE Transactions on Medical Imaging·Josien P W PluimMax A Viergever
Jan 11, 2007·Journal of Microscopy·S Bolte, F P Cordelières
Sep 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Erin H Seeley, Richard M Caprioli
Mar 19, 2011·Rapid Communications in Mass Spectrometry : RCM·John S FletcherJohn C Vickerman
Apr 3, 2012·Analytical Chemistry·Dominic J HarePhilip A Doble
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Mar 8, 2013·Biochimica Et Biophysica Acta·Herbert ThielePeter Maass
Mar 4, 2014·Nature Methods·Charlotte GiesenBernd Bodenmiller
Apr 29, 2015·Journal of the American Society for Mass Spectrometry·Karolina ŠkráškováRon M A Heeren
Jan 13, 2016·Analytical and Bioanalytical Chemistry·Alexander Gundlach-Graham, Detlef Günther
Feb 7, 2018·The Journal of Pathology·Raúl CatenaBernd Bodenmiller

❮ Previous
Next ❯

Citations

May 9, 2021·Nuclear Medicine and Biology·Ines M CostaSamantha Y A Terry
Jun 15, 2021·Chemical Science·Elizabeth J AnthonyZijin Zhang
Dec 8, 2020·Analytical Chemistry·Keke HuAndrew G Ewing
Dec 1, 2021·Analytical Chemistry·Anna SchoeberlGunda Koellensperger

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.