Three-dimensional scanless holographic optogenetics with temporal focusing (3D-SHOT)

Nature Communications
Nicolas C PégardHillel Adesnik

Abstract

Optical methods capable of manipulating neural activity with cellular resolution and millisecond precision in three dimensions will accelerate the pace of neuroscience research. Existing approaches for targeting individual neurons, however, fall short of these requirements. Here we present a new multiphoton photo-excitation method, termed three-dimensional scanless holographic optogenetics with temporal focusing (3D-SHOT), which allows precise, simultaneous photo-activation of arbitrary sets of neurons anywhere within the addressable volume of a microscope. This technique uses point-cloud holography to place multiple copies of a temporally focused disc matching the dimensions of a neuron's cell body. Experiments in cultured cells, brain slices, and in living mice demonstrate single-neuron spatial resolution even when optically targeting randomly distributed groups of neurons in 3D. This approach opens new avenues for mapping and manipulating neural circuits, allowing a real-time, cellular resolution interface to the brain.

References

Apr 6, 1990·Science·W DenkW W Webb
Aug 22, 1989·The Journal of Comparative Neurology·A Schüz, G Palm
Aug 24, 2005·Nature Neuroscience·Edward S BoydenKarl Deisseroth
Oct 30, 2007·Nature Methods·Volodymyr NikolenkoRafael Yuste
Dec 24, 2008·Optics Express·Eirini PapagiakoumouValentina Emiliani
Jan 9, 2009·Frontiers in Neural Circuits·Volodymyr NikolenkoRafael Yuste
Jan 23, 2009·Nature Methods·Christoph LutzValentina Emiliani
Mar 21, 2005·Optics Express·Guanghao ZhuChris Xu
Dec 11, 2006·Optics Express·Michael E DurstChris Xu
Feb 19, 2007·Optics Express·Roberto Di LeonardoGiancarlo Ruocco
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·John Peter Rickgauer, David W Tank
Oct 20, 2009·Journal of Neural Engineering·L GolanS Shoham
Apr 24, 2010·Nature·Hillel Adesnik, Massimo Scanziani
Jun 15, 2010·Proceedings of the National Academy of Sciences of the United States of America·Bertalan K AndrasfalvyAlipasha Vaziri
Sep 21, 2010·Nature Methods·Eirini PapagiakoumouValentina Emiliani
Jun 23, 2011·Annual Review of Neuroscience·Lief FennoKarl Deisseroth
Aug 25, 2011·Nature Communications·David J McCabeBéatrice Chatel
Jan 3, 2012·Current Opinion in Neurobiology·Alipasha Vaziri, Valentina Emiliani
Nov 13, 2012·Nature Methods·Adam M PackerRafael Yuste
Nov 22, 2012·Nature Methods·Rohit PrakashKarl Deisseroth
Oct 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·Hanno S MeyerMarcel Oberlaender
Feb 11, 2014·Nature Methods·Nathan C KlapoetkeEdward S Boyden
Nov 2, 2014·Optics Letters·Oscar HernandezValentina Emiliani
Nov 18, 2014·Nature Neuroscience·John Peter RickgauerDavid W Tank
Mar 6, 2015·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Kelly B ClancyDaniel E Feldman
Sep 29, 2015·Nature Neuroscience·Scott PlutaHillel Adesnik
Aug 16, 2016·Science·Luis Carrillo-ReidRafael Yuste
Nov 1, 2016·Nature Methods·Robert PrevedelAlipasha Vaziri

❮ Previous
Next ❯

Citations

May 2, 2018·Nature Neuroscience·Alan R MardinlyHillel Adesnik
Mar 21, 2019·Frontiers in Cellular Neuroscience·Peter QuickeThomas Knöpfel
Apr 4, 2020·Annual Review of Biomedical Engineering·Sheng LiuFang Huang
May 29, 2018·Nature Neuroscience·Adam E Cohen, Samouil L Farhi
Dec 25, 2019·Applied Optics·Alejandro Velez-Zea, Roberto Torroba
Apr 15, 2020·Nature Methods·Eirini PapagiakoumouValentina Emiliani
May 24, 2018·Applied Optics·Ryoichi HorisakiJun Tanida
Oct 20, 2018·Science Advances·Adrià Escobet-MontalbánKishan Dholakia
Sep 11, 2020·Optics Express·M Hossein EybposhNicolas C Pégard
Feb 23, 2020·Scientific Reports·Priya S BalasubramanianAmit Lal
Nov 14, 2018·Nature Methods·Zihui ZhangMichael Häusser
May 7, 2019·Journal of Physics D: Applied Physics·Navjeevan S SoorAmanda J Foust
Jan 9, 2019·Frontiers in Cellular Neuroscience·Emiliano RonzittiEirini Papagiakoumou
Feb 26, 2019·Nature Protocols·Ido CarmiShai Berlin
Nov 4, 2020·PLoS Biology·Jonathan Andrew CheungSamuel Andrew Hires
Jan 4, 2021·Current Opinion in Neurobiology·Brian M SweisDenise J Cai
Jun 11, 2020·Cell Reports·Nicole A RepinaDavid V Schaffer
Jan 28, 2021·Journal of the American Chemical Society·Monica A GonzalezEvan W Miller
Dec 11, 2020·Light, Science & Applications·Nathan Tessema ErsumoRikky Muller
Mar 11, 2021·Applied Optics·Alejandro Velez-Zea, Roberto Torroba
Aug 17, 2020·Journal of Neuroscience Methods·Brice De La CrompeIlka Diester
Apr 11, 2018·Current Opinion in Neurobiology·I-Wen ChenValentina Emiliani
Apr 17, 2018·Current Opinion in Neurobiology·Weijian Yang, Rafael Yuste
Mar 16, 2021·Frontiers in Cellular Neuroscience·Paolo Pozzi, Jonathan Mapelli
Aug 18, 2021·Nature Neuroscience·Hillel Adesnik, Lamiae Abdeladim
Feb 23, 2019··Philip WijesingheAdrià Escobet-Montalbán

❮ Previous
Next ❯

Methods Mentioned

BETA
sedation

Software Mentioned

Mathworks
Matlab
3D
SHOT

Related Concepts

Related Feeds

BioHub - Researcher Network

The Chan-Zuckerberg Biohub aims to support the fundamental research and develop the technologies that will enable physicians to cure, prevent, or manage all diseases in our childrens' lifetimes. The CZ Biohub brings together researchers from UC Berkeley, Stanford, and UCSF. Find the latest research from the CZ Biohub researcher network here.