Three-dimensional scoring of zebrafish behavior unveils biological phenomena hidden by two-dimensional analyses

Scientific Reports
Simone MacrìMaurizio Porfiri

Abstract

The study of zebrafish behavior represents a cornerstone upon which basic researchers promise to advance knowledge in life sciences. Although zebrafish swim in a three-dimensional (3D) space, their behavior in the lab is almost exclusively scored in two dimensions, whereby zebrafish are recorded using a single camera providing 2D videos. Whether this dimensional reduction preserves the reliability of data has not been addressed. Here we show that, compared to a 3D observation, 2D data are flawed by over-reporting and under-reporting of locomotory differences. Specifically, we first reconstructed 3D trajectories through the integration of synchronous information derived from two cameras, and then compared them with the original 2D views in classical experimental paradigms assessing shoaling tendency, fear, anxiety, and general locomotion. Our results suggest that traditional behavioral scoring of individual zebrafish performed in 2D may undermine data integrity, thereby requiring a general reconsideration of scoring zebrafish behavior to incorporate a 3D approach. We then demonstrate that, compared to 2D, a 3D approach requires a reduced number of subjects to achieve the same degree of validity. We anticipate these findings to l...Continue Reading

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Citations

Jan 14, 2018·Scientific Reports·Changsu KimMaurizio Porfiri
Sep 12, 2019·Journal of the Royal Society, Interface·Giovanni PolverinoMaurizio Porfiri
Nov 16, 2018·Biological cybernetics·Donato RomanoCesare Stefanini
Aug 16, 2019·Disease Models & Mechanisms·Yijie Geng, Randall T Peterson
Aug 12, 2020·Lab Animal·Simone MacrìMaurizio Porfiri
Feb 5, 2021·Frontiers in Behavioral Neuroscience·William JooSummer B Thyme
Aug 14, 2019·Frontiers in Robotics and AI·Yanpeng YangMaurizio Porfiri
Nov 15, 2020·Progress in Neuro-psychopharmacology & Biological Psychiatry·Mert KarakayaMaurizio Porfiri
May 4, 2020·Progress in Neuro-psychopharmacology & Biological Psychiatry·Luiz V RosaDenis B Rosemberg

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