Three-dimensional solution structure and stability of phage 434 Cro protein

Biochemistry
S PadmanabhanM Rico

Abstract

1H NMR resonances of the phage 434 Cro protein were assigned using standard 2D NMR methods, and its solution structure determined using 867 distance constraints in distance geometry (DIANA) calculations ultimately refined by restrained molecular dynamics (GROMOS). In the 20 best NMR structures, the average pairwise backbone and heavy atom RMSDs are 0.63 +/- 0.14 and 1.53 +/- 0.15 A, respectively, for the structurally well-defined residues 4-65. Residues 1-3 and 66-71 at the N- and C-termini are structurally disordered. The region 4-65 includes five alpha-helices and tight turns which define the hydrophobic core of the protein. The backbone and heavy atom RMSDs for residues 4-65 are 0.92 +/- 0.12 and 1.99 +/- 0.12 A, respectively, for the NMR versus the crystal structures, but there are significant differences in the side-chain conformations and solvent accessibilities for some core residues. Analytical ultracentrifugation experiments confirm that 434 Cro is monomeric even at the high NMR concentrations. 434 Cro folding under NMR solution conditions is two-state as indicated by coincident urea denaturation curves from circular dichroism and intrinsic fluorescence measurements. They yield values for 434 Cro stability which show g...Continue Reading

References

Jan 1, 1977·Annual Review of Biophysics and Bioengineering·F M Richards
Nov 20, 1991·Journal of Molecular Biology·D S WishartF M Richards
May 20, 1991·Journal of Molecular Biology·A Mondragón, S C Harrison
Jan 1, 1990·Annual Review of Biochemistry·S C Harrison, A K Aggarwal
Feb 1, 1996·Journal of Molecular Graphics·R KoradiK Wüthrich
Jan 20, 1965·Journal of the American Chemical Society·G HOLZWARTH, P DOTY
Jul 1, 1995·Journal of Biomolecular NMR·C BartelsK Wüthrich

❮ Previous
Next ❯

Citations

Jan 8, 2005·Chembiochem : a European Journal of Chemical Biology·Reinhard ReentsHerbert Waldmann
Mar 31, 1998·Current Opinion in Structural Biology·J Clarke, L S Itzhaki
Jul 6, 2006·Nucleic Acids Research·Marc van DijkAlexandre M J J Bonvin
Aug 19, 1999·Protein Science : a Publication of the Protein Society·S PadmanabhanM Rico
Feb 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Kelly R LeFevre, Matthew H J Cordes
Jan 31, 2003·Proteins·Carlos GonzálezFrancese X Avilés
Apr 6, 2004·Structure·Tracey NewloveMatthew H J Cordes
Nov 16, 2018·Journal of the American Chemical Society·Miguel Ángel TreviñoDouglas V Laurents

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.