Three-dimensional solution structure of a single zinc finger DNA-binding domain

Science
M S LeeP E Wright

Abstract

The three-dimensional solution structure of a zinc finger nucleic acid binding motif has been determined by nuclear magnetic resonance (NMR) spectroscopy. Spectra of a synthetic peptide corresponding to a single zinc finger from the Xenopus protein Xfin yielded distance and dihedral angle constraints that were used to generate structures from distance geometry and restrained molecular dynamics calculations. The zinc finger is an independently folded domain with a compact globular structure in which the zinc atom is bound by two cysteine and two histidine ligands. The polypeptide backbone fold consists of a well-defined helix, starting as alpha and ending as 3(10) helix, packed against two beta strands that are arranged in a hairpin structure. A high density of basic and polar amino acid side chains on the exposed face of the helix are probably involved in DNA binding.

References

Sep 16, 1988·Science·G PárragaR E Klevit
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J M Berg
Jan 15, 1988·Cell·R M Evans, S M Hollenberg
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A D FrankelC O Pabo
Apr 1, 1986·Journal of Computational Chemistry·Scott J WeinerDavid A Case

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Citations

Jun 1, 1993·Protein Science : a Publication of the Protein Society·M J Sutcliffe
Jun 1, 1993·Protein Science : a Publication of the Protein Society·R C HoffmanR E Klevit
Aug 1, 1993·Protein Science : a Publication of the Protein Society·B A KrizekJ M Berg
Nov 1, 1993·Proteins·M W MacArthur, J M Thornton
Aug 1, 1994·Proteins·Q Zheng, D J Kyle
May 16, 2001·Biochemical and Biophysical Research Communications·W ZhangX Cao
Dec 1, 1994·Journal of Molecular Evolution·L C ShimminW H Li
Apr 1, 1991·Journal of Molecular Evolution·J Lanfear, P W Holland
Apr 25, 2012·Journal of Biomolecular NMR·Pierre BarraudFrédéric H-T Allain
Feb 21, 2008·Cell Biochemistry and Biophysics·Kathryn J BrayerDavid J Segal
Feb 7, 2008·Cell Biochemistry and Biophysics·Kathryn J Brayer, David J Segal
Dec 1, 1992·The International Journal of Biochemistry·W M Krajewska
Jan 1, 1991·Progress in Biophysics and Molecular Biology·B S Shastry
Jan 1, 1995·Progress in Biophysics and Molecular Biology·R ParthasarathyK Go
Sep 1, 1994·Computers & Chemistry·J Heringa
Oct 1, 1993·International Journal of Biological Macromolecules·M GielG Kupryszewski
Dec 1, 1989·Trends in Pharmacological Sciences·F J Zeelen
May 1, 1996·Brain Research. Molecular Brain Research·U PottM E Schwab
Nov 1, 1991·Mechanisms of Development·T el-Baradi, T Pieler
Aug 1, 1991·Current Opinion in Biotechnology·J R Coggins
Aug 1, 1991·Current Opinion in Biotechnology·J A TainerE D Getzoff
Mar 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·K Dahlman-WrightJ A Gustafsson
Jun 1, 1996·Bioorganic & Medicinal Chemistry·C L Nesloney, J W Kelly
Aug 3, 2013·Analytical Biochemistry·Sanela MarticHeinz-Bernhard Kraatz
Nov 26, 2003·Journal of Molecular Biology·Javier Fernández-MartínezEduardo A Espeso
Jul 25, 2003·Toxicology and Applied Pharmacology·Nasser H Zawia
Jan 2, 2001·Journal of Inorganic Biochemistry·M Nagaoka, Y Sugiura
May 29, 1998·Biochimica Et Biophysica Acta·T MiuraH Takeuchi

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