PMID: 9177168Jun 10, 1997Paper

Three-dimensional solution structure of the complex of alpha-bungarotoxin with a library-derived peptide

Proceedings of the National Academy of Sciences of the United States of America
Tali ScherfJ Anglister

Abstract

The solution structure of the complex between alpha-bungarotoxin (alpha-BTX) and a 13-residue library-derived peptide (MRYYESSLKSYPD) has been solved using two-dimensional proton-NMR spectroscopy. The bound peptide adopts an almost-globular conformation resulting from three turns that surround a hydrophobic core formed by Tyr-11 of the peptide. The peptide fills an alpha-BTX pocket made of residues located at fingers I and II, as well as at the C-terminal region. Of the peptide residues, the largest contact area is formed by Tyr-3 and Tyr-4. These findings are in accord with the previous data in which it had been shown that substitution of these aromatic residues by aliphatic amino acids leads to loss of binding of the modified peptide with alpha-BTX. Glu-5 and Leu-8, which also remarkably contribute to the contact area with the toxin, are present in all the library-derived peptides that bind strongly to alpha-BTX. The structure of the complex may explain the fact that the library-derived peptide binds alpha-BTX with a 15-fold higher affinity than that shown by the acetylcholine receptor peptide (alpha185-196). Although both peptides bind to similar sites on alpha-BTX, the latter adopts an extended conformation when bound to th...Continue Reading

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Citations

Feb 27, 2001·Toxicon : Official Journal of the International Society on Toxinology·M BalassE Katchalski-Katzir
Jul 27, 1999·Combinatorial Chemistry & High Throughput Screening·G CesareniG Cestra
Sep 22, 1999·European Journal of Biochemistry·V Tsetlin
Jun 7, 2001·Proceedings of the National Academy of Sciences of the United States of America·T ScherfE Katchalski-Katzir
Nov 1, 2003·Annals of the New York Academy of Sciences·Sara FuchsEphraim Katchalski-Katzir
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·M BalassS Fuchs
Mar 17, 2001·Chemistry & Biology·R KasherE Katchalski-Katzir

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