Three-dimensional spheroid cell model of in vitro adipocyte inflammation

Tissue Engineering. Part a
Paul A TurnerAmol V Janorkar

Abstract

To improve treatment of obesity, a contributing factor to multiple systemic and metabolic diseases, a better understanding of metabolic state and environmental stress at the cellular level is essential. This work presents development of a three-dimensional (3D) in vitro model of adipose tissue displaying induced lipid accumulation as a function of fatty acid supplementation that, subsequently, investigates cellular responses to a pro-inflammatory stimulus, thereby recapitulating key stages of obesity progression. Three-dimensional spheroid organization of adipose cells was induced by culturing 3T3-L1 mouse preadipocytes on an elastin-like polypeptide-polyethyleneimine (ELP-PEI)-coated surface. Results indicate a more differentiated phenotype in 3D spheroid cultures relative to two-dimensional (2D) monolayer analogues based on triglyceride accumulation, CD36 and CD40 protein expression, and peroxisome proliferator-activated receptor-γ (PPAR-γ) and adiponectin mRNA expression. The 3T3-L1 adipocyte spheroid model was then used to test the effects of a pro-inflammatory microenvironment, namely maturation in the presence of elevated fatty acid levels followed by acute exposure to tumor necrosis factor alpha (TNF-α). Under these cond...Continue Reading

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Citations

Oct 2, 2015·Tissue Engineering. Part a·Johnna S Temenoff
May 21, 2016·Advanced Healthcare Materials·Rosalyn D AbbottDavid L Kaplan
Jul 5, 2016·International Journal of Molecular Sciences·Francisco Javier Ruiz-OjedaConcepción María Aguilera
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Jan 1, 2019·BMC Biomedical Engineering·Connor S MurphyMichaela R Reagan
May 12, 2020·Advanced Biosystems·Alireza Shahin-Shamsabadi, Ponnambalam Ravi Selvaganapathy
May 13, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Sarah J FitzgeraldAmol V Janorkar
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Sep 7, 2020·Differentiation; Research in Biological Diversity·Muhammad Sufyan VohraEng Hwa Wong
Apr 4, 2021·International Journal of Molecular Sciences·María Gómez-SerranoElke Pogge von Strandmann
Jan 14, 2021·American Journal of Physiology. Cell Physiology·Jérémy DufauDominique Langin
Jun 25, 2021·Advanced Science·Joanne X ShenVolker M Lauschke
Jul 22, 2021·Adipocyte·Chitmandeep JosanSandeep Raha
Jun 29, 2018·ACS Applied Materials & Interfaces·Eunsol KimGiyoong Tae

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Methods Mentioned

BETA
protein assay
ELISA
fatty

Software Mentioned

ImageJ

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