Three-dimensional spheroid culture of human umbilical cord mesenchymal stem cells promotes cell yield and stemness maintenance

Cell and Tissue Research
Yi LiHong Bu

Abstract

Mesenchymal stem cell (MSC) transplantation is a promising treatment of many diseases. However, conventional techniques with cells being cultured as a monolayer result in slow cell proliferation and insufficient yield to meet clinical demands. Three-dimensional (3D) culture systems are gaining attention with regard to recreating a complex microenvironment and to understanding the conditions experienced by cells. Our aim is to establish a novel 3D system for the culture of human umbilical cord MSCs (hUC-MSCs) within a real 3D microenvironment but with no digestion or passaging. Primary hUC-MSCs were isolated and grown in serum-free medium (SFM) on a suspension Rocker system. Cell characteristics including proliferation, phenotype and multipotency were recorded. The therapeutic effects of 3D-cultured hUC-MSCs on carbon tetrachloride (CCl4)-induced acute liver failure in mouse models were examined. In the 3D Rocker system, hUC-MSCs formed spheroids in SFM and maintained high viability and active proliferation. Compared with monolayer culture, the 3D-culture system yielded more hUC-MSCs cells within the same volume. The spheroids expressed higher levels of stem cell markers and displayed stronger multipotency. After transplantation...Continue Reading

References

Apr 2, 1999·Science·M F PittengerD R Marshak
Sep 16, 2000·Experimental Hematology·R J Deans, A B Moseley
Dec 12, 2002·Molecular Biology of the Cell·Patricia A ZukMarc H Hedrick
Apr 29, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jong-Seop RimBarbara Gawronska-Kozak
Jan 24, 2007·Stem Cell Reviews·Mark L Weiss, Deryl L Troyer
Aug 16, 2008·Stem Cells·Mark L WeissKevin R McIntosh
May 8, 2010·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Roberto Francese, Paolo Fiorina
Aug 13, 2011·Cell and Tissue Research·Priya R Baraniak, Todd C McDevitt
Oct 18, 2011·Biologicals : Journal of the International Association of Biological Standardization·Carol Marcus-SekuraRebecca L Sheets
May 23, 2012·Journal of Tissue Engineering and Regenerative Medicine·Mai T Lam, Michael T Longaker
May 14, 2014·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Panagiotis KrontirasDaniel A Hägg

❮ Previous
Next ❯

Citations

Apr 26, 2016·Acta Biomaterialia·Shu-Pei QiaoWei-Ming Tian
Dec 6, 2018·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·C J Cooksey
Mar 7, 2020·Systems Biology in Reproductive Medicine·Marzieh Ziloochi KashaniFereshteh Mehraein
Aug 1, 2020·Advanced Healthcare Materials·Se-Jeong KimHeungsoo Shin
Apr 28, 2017·Stem Cell Research & Therapy·Yuriy PetrenkoŠárka Kubinová
Jul 1, 2017·Applied Biochemistry and Biotechnology·Diandian ZhaoHonggang Hu
Jul 19, 2020·Stem Cell Reviews and Reports·Aleksandra JaukovićMilena Mourdjeva
Jan 25, 2019·Journal of Biological Engineering·Andrew HamannAngela K Pannier
Nov 26, 2020·Scientific Reports·Brent M BijonowskiSamuel C Grant
Sep 21, 2020·Journal of Endodontics·Carolina CuccoJacques E Nör
Dec 29, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Farid N FaruquKhuloud T Al-Jamal
Mar 2, 2021·Frontiers in Bioengineering and Biotechnology·Dimitrios Kouroupis, Diego Correa

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.