Three-dimensional structure of a fluorescein-Fab complex crystallized in 2-methyl-2,4-pentanediol

Proteins
J N HerronA B Edmundson

Abstract

The crystal structure of a fluorescein-Fab (4-4-20) complex was determined at 2.7 A resolution by molecular replacement methods. The starting model was the refined 2.7 A structure of unliganded Fab from an autoantibody (BV04-01) with specificity for single-stranded DNA. In the 4-4-20 complex fluorescein fits tightly into a relatively deep slot formed by a network of tryptophan and tyrosine side chains. The planar xanthonyl ring of the hapten is accommodated at the bottom of the slot while the phenylcarboxyl group interfaces with solvent. Tyrosine 37 (light chain) and tryptophan 33 (heavy chain) flank the xanthonyl group and tryptophan 101 (light chain) provides the floor of the combining site. Tyrosine 103 (heavy chain) is situated near the phenyl ring of the hapten and tyrosine 102 (heavy chain) forms part of the boundary of the slot. Histidine 31 and arginine 39 of the light chain are located in positions adjacent to the two enolic groups at opposite ends of the xanthonyl ring, and thus account for neutralization of one of two negative charges in the haptenic dianion. Formation of an enol-arginine ion pair in a region of low dielectric constant may account for an incremental increase in affinity of 2-3 orders of magnitude in ...Continue Reading

References

Jul 1, 1977·Immunochemistry·R M Watt, E W Voss
May 1, 1976·Immunochemistry·E W VossR T Root
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S SheriffD R Davies
Jun 12, 1989·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·A B EdmundsonE W Voss
Oct 21, 1988·Science·R E BirdM Whitlow
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·L M AmzelF F Richards
Nov 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·D M SegalD R Davies

❮ Previous
Next ❯

Citations

Jun 1, 1993·Journal of Molecular Recognition : JMR·E W Voss
Nov 26, 2002·Journal of Molecular Recognition : JMR·Allen B Edmundson
Nov 26, 2002·Journal of Molecular Recognition : JMR·Ai-Ping Wei, James N Herron
Feb 1, 1993·Protein Science : a Publication of the Protein Society·J F SchillbachM N Margolies
May 1, 1994·Protein Science : a Publication of the Protein Society·J F SchildbachR I Near
Mar 19, 1999·Protein Science : a Publication of the Protein Society·C L CasipitH C Wong
Jan 1, 1992·Progress in Biophysics and Molecular Biology·J Novotny, K Sharp
Oct 1, 1989·Molecular Immunology·E W VossD W Ballard
Feb 1, 1994·Molecular Immunology·E A Padlan
Nov 1, 1995·Molecular Immunology·M E Mummert, E W Voss
Jun 1, 1996·Molecular Immunology·K D WhiteF J Waxman
Apr 1, 1991·Trends in Biotechnology·R E Bird, B W Walker
Feb 1, 1991·Current Opinion in Immunology
Apr 28, 1995·Journal of Molecular Biology·P D JeffreyS Sheriff
Sep 1, 1996·Immunotechnology : an International Journal of Immunological Engineering·A PopeK S Johnson
May 7, 1999·Immunotechnology : an International Journal of Immunological Engineering·H de HaardB van Gemen
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·V A RobertsJ A Tainer
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·C F BarbasR A Lerner
Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R K GanjuE L Reinherz
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L W GuddatA B Edmundson
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·M A RoguskaB C Guild
Jan 1, 1997·Annual Review of Biophysics and Biomolecular Structure·H Wade, T S Scanlan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.