Three-dimensional structure of neuropeptide k bound to dodecylphosphocholine micelles

Biochemistry
Anjali Dike, Sudha M Cowsik

Abstract

Neuropeptide K (NPK), an N-terminally extended form of neurokinin A (NKA), represents the most potent and longest lasting vasodepressor and cardiomodulatory tachykinin reported thus far. NPK has been shown to have high selectivity for the NK2 receptor. Because the micelle-associated structure may be relevant to the NPK-receptor interaction, the three-dimensional structure of the NPK in aqueous and micellar environments has been studied by two-dimensional proton nuclear magnetic resonance (2D (1)H NMR spectroscopy) and distance geometry calculations. Proton NMR assignments have been carried out with the aid of correlation spectroscopy (DQF-COSY and TOCSY) and nuclear Overhauser effect spectroscopy (NOESY and ROESY) experiments. The interproton distances and dihedral angle constraints obtained from the NMR data have been used in torsion angle dynamics algorithm for NMR applications (DYANA) to generate a family of structures, which have been refined using restrained energy minimization and dynamics. The results show that in an aqueous environment NPK lacks a definite secondary structure, although some turn-like elements are present in the N terminus. The structure is well-defined in the presence of dodecylphosphocholine micelles. ...Continue Reading

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Feb 26, 2005·Biophysical Journal·Anjali Dike, Sudha M Cowsik

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Citations

Dec 21, 2007·Journal of Biomolecular Structure & Dynamics·Anjali Dike, Sudha M Cowsik
May 11, 2010·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·David N Langelaan, Jan K Rainey
May 5, 2009·Journal of Structural Biology·Anjali D Ganjiwale, Sudha M Cowsik
Aug 25, 2015·Biopolymers·Anjali Ganjiwale, Sudha M Cowsik
Sep 19, 2006·Journal of Structural Biology·Anjali Dike, Sudha M Cowsik
Nov 6, 2014·Biomolecular Concepts·Takenori Onaga

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