Three-dimensional structure of respiratory complex I from Escherichia coli in ice in the presence of nucleotides.

Biochimica Et Biophysica Acta
David J Morgan, Leonid A Sazanov

Abstract

Complex I (NADH:ubiquinone oxidoreductase) is the largest protein complex of bacterial and mitochondrial respiratory chains. The first three-dimensional structure of bacterial complex I in vitrified ice was determined by electron cryo-microscopy and single particle analysis. The structure of the Escherichia coli enzyme incubated with either NAD(+) (as a reference) or NADH was calculated to 35 and 39 A resolution, respectively. The X-ray structure of the peripheral arm of Thermus thermophilus complex I was docked into the reference EM structure. The model obtained indicates that Fe-S cluster N2 is close to the membrane domain interface, allowing for effective electron transfer to membrane-embedded quinone. At the current resolution, the structures in the presence of NAD(+) or NADH are similar. Additionally, side-view class averages were calculated for the negatively stained bovine enzyme. The structures of bovine complex I in the presence of either NAD(+) or NADH also appeared to be similar. These observations indicate that conformational changes upon reduction with NADH, suggested to occur by a range of studies, are smaller than had been thought previously. The model of the entire bacterial complex I could be built from the cry...Continue Reading

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Citations

May 28, 2010·Nature·Rouslan G EfremovLeonid A Sazanov
Jan 6, 2011·Proceedings of the National Academy of Sciences of the United States of America·Clément AuriolIsabelle Meynial-Salles
Aug 13, 2011·The Journal of Biological Chemistry·Jesus Torres-BaceteTakao Yagi
Mar 4, 2010·The Plant Cell·Jennifer KlodmannHans-Peter Braun
May 10, 2011·Annual Review of Biochemistry·Nelson P Barrera, Carol V Robinson
Jan 26, 2016·Biochimica Et Biophysica Acta·John M BerrisfordLeonid A Sazanov
Nov 28, 2015·Pathogens and Disease·Cristina Uribe-AlvarezSalvador Uribe-Carvajal
Aug 13, 2011·Current Opinion in Structural Biology·Rouslan G Efremov, Leonid A Sazanov
Dec 21, 2010·Phytochemistry·Jennifer Klodmann, Hans-Peter Braun
Apr 16, 2009·Biochimica Et Biophysica Acta·Volker ZickermannUlrich Brandt
Dec 23, 2009·The Biochemical Journal·Judy Hirst
Feb 2, 2010·Biochimica Et Biophysica Acta·Maja A TocilescuUlrich Brandt
May 25, 2010·Biochimica Et Biophysica Acta·Maja A TocilescuUlrich Brandt
May 19, 2009·Biochimica Et Biophysica Acta·Vernon A CouchAlexei A Stuchebrukhov
Oct 16, 2016·Protein & Cell·Runyu GuoMaojun Yang
May 21, 2015·Nature Reviews. Molecular Cell Biology·Leonid A Sazanov
Jan 13, 2011·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Ruth HielscherPetra Hellwig
Dec 1, 2010·The Journal of Biological Chemistry·Chui-ying YipLeonid A Sazanov
Mar 9, 2021·The Plant Journal : for Cell and Molecular Biology·Sofia Shevtsov-TalOren Ostersetzer-Biran

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