Three-dimensional structure of Rubella virus factories.

Virology
Juan FontanaCristina Risco

Abstract

Viral factories are complex structures in the infected cell where viruses compartmentalize their life cycle. Rubella virus (RUBV) assembles factories by recruitment of rough endoplasmic reticulum (RER), mitochondria and Golgi around modified lysosomes known as cytopathic vacuoles or CPVs. These organelles contain active replication complexes that transfer replicated RNA to assembly sites in Golgi membranes. We have studied the structure of RUBV factory in three dimensions by electron tomography and freeze-fracture. CPVs contain stacked membranes, rigid sheets, small vesicles and large vacuoles. These membranes are interconnected and in communication with the endocytic pathway since they incorporate endocytosed BSA-gold. RER and CPVs are coupled through protein bridges and closely apposed membranes. Golgi vesicles attach to the CPVs but no tight contacts with mitochondria were detected. Immunogold labelling confirmed that the mitochondrial protein p32 is an abundant component around and inside CPVs where it could play important roles in factory activities.

References

Dec 1, 1988·The Journal of Cell Biology·S FroshauerA Helenius
Sep 1, 1972·Journal of Virology·P M GrimleyR M Friedman
Jan 1, 1994·Advances in Virus Research·T K Frey
Apr 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C Risco, P Pinto da Silva
Jan 1, 1996·Journal of Structural Biology·J R KremerJ R McIntosh
May 20, 1998·Journal of Microscopy·C Risco, P Pinto da Silva
Mar 27, 2001·Journal of Virology·P KujalaL Kääriäinen
Dec 28, 2002·Journal of Virology·Iñigo J SalanuevaCristina Risco
Mar 12, 2003·Trends in Cell Biology·Alasdair C Steven, Ueli Aebi
May 22, 2003·Journal of Cell Science·Ansgar SantelMargaret T Fuller
Aug 16, 2003·Virology·Cristina RiscoTeryl K Frey
Nov 25, 2003·Science·Kay GrünewaldAlasdair C Steven
Oct 1, 2004·Molecular Biology of the Cell·Melanie L StyersVictor Faundez
Dec 2, 2004·Journal of Cell Science·Naotada IshiharaKatsuyoshi Mihara
Jan 18, 2005·Trends in Cell Biology·Richard McIntoshDavid Mastronarde
Jan 20, 2005·Biology of the Cell·Reyes R NovoaCristina Risco
Feb 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Marek CyrklaffJosé L Carrascosa
Apr 9, 2005·Journal of Microscopy·J C Fiala
Jun 15, 2005·Annual Review of Biochemistry·Vladan LucićWolfgang Baumeister
Jun 21, 2005·Journal of Structural Biology·Giovanni CardoneAlasdair C Steven
Sep 30, 2005·Traffic·Jason Mackenzie
May 25, 2006·Annual Review of Microbiology·Juan Ortín, Francisco Parra
Jun 27, 2006·Cell Biology International·Pilar Cabezas, Cristina Risco
Sep 20, 2006·The Journal of Cell Biology·György CsordásGyörgy Hajnóczky
Nov 8, 2006·Cellular Microbiology·Juan FontanaCristina Risco
Jan 17, 2007·Journal of Structural Biology·Peter van der HeideNiels Volkmann
Apr 5, 2007·Nature Protocols·Nicholas J Severs
Sep 4, 2007·Advances in Virus Research·Christopher NethertonThomas Wileman
Sep 18, 2007·Journal of Structural Biology·J Bernard HeymannAlasdair C Steven
Nov 7, 2007·The Biochemical Journal·Ho Lam TangMing Chiu Fung
Feb 5, 2008·Cell Calcium·Kirill Kiselyov, Shmuel Muallem
Apr 17, 2008·Nature Reviews. Microbiology·Sven Miller, Jacomine Krijnse-Locker
Apr 22, 2008·Annual Review of Phytopathology·Peter D Nagy
May 16, 2008·PLoS Biology·Nathan ZaubermanAbraham Minsky
Jun 13, 2008·Cellular Microbiology·Juan FontanaCristina Risco

❮ Previous
Next ❯

Citations

Jan 13, 2012·The Journal of General Virology·Suganthi SuppiahTeryl K Frey
Dec 5, 2014·PLoS Pathogens·Mathieu DubéMargaret Kielian
Feb 10, 2018·Viruses·Farhana Abu Bakar, Lisa F P Ng
Dec 27, 2018·Cellular Microbiology·Alberto Fernández-OlivaCristina Risco
Jul 24, 2014·Viruses·Inés Romero-Brey, Ralf Bartenschlager
Sep 18, 2014·MBio·Emily A DesmetJohn S L Parker
May 2, 2020·The Journal of Cell Biology·Isabel Fernández de CastroCristina Risco
May 28, 2020·International Journal of Molecular Sciences·Shanhui RenYingjie Sun
Nov 3, 2014·Annual Review of Virology·Cristina RiscoSriram Subramaniam
Sep 18, 2012·Cellular Microbiology·Isabel Fernández de CastroCristina Risco
Jun 25, 2016·Viruses·Inés Romero-Brey, Ralf Bartenschlager
Feb 3, 2021·Viruses·Georg Wolff, Montserrat Bárcena
Feb 26, 2021·Journal of Virology·Pratyush Kumar Das, Margaret Kielian

❮ Previous
Next ❯

Methods Mentioned

BETA
electron microscopy
chemical treatments
electron tomography
light
FCS
in
transfection

Software Mentioned

AMIRA
FEI
IMOD
Bsoft

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.