Three-dimensional structure of the Chinese Sacbrood bee virus

Science in China. Series C, Life Sciences
J ZhangX Lu

Abstract

The RNA of Chinese Sacbrood Bee Virus (CSBV) was purified and used as template to obtain a 1096 bp cDNA fragment by RT-PCR amplification. This DNA fragment was cloned into pGEM-T Easy Vector for sequencing. Analyses of the sequenced CSBV RNA fragment revealed a nucleotide sequence homology of 87.6% and a deduced amino-acid sequence homology of 94.6% with that of the Sacbrood Virus (SBV), indicating that CSBV is a different but highly homologous virus of SBV. The three-dimensional (3D) structure of CSBV was determined at 2.5 nm resolution by using electron cryo-microscopy (cryoEM) and computer reconstruction methods. The 3-D structure showed that the capsid has aT = 1 (or P = 3) icosahedral capsid shell with a smooth surface. There were 12 pentons at its icosahedral vertices (5-fold axes) and 132 holes penetrating the shell. The 3-D structure also revealed densities corresponding to the CSBV genome, suggesting icosahedrally-ordered RNA organization, a novel feature not previously reported for any picornaviruses.

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Citations

Mar 22, 2014·Journal of Invertebrate Pathology·J M K Roberts, D L Anderson
Apr 10, 2010·Current Microbiology·Xuejiao LiuRichou Han
Dec 18, 2009·Science in China. Series C, Life Sciences·Li XieXuePing Zhou
Mar 24, 2015·Journal of Veterinary Science·Ah-Jin AhnSung-Shik Shin
Mar 9, 2017·Proceedings of the National Academy of Sciences of the United States of America·Karel ŠkubníkPavel Plevka
Jun 10, 2016·Journal of Virology·Sergei KalynychPavel Plevka
Jul 11, 2018·Proceedings of the National Academy of Sciences of the United States of America·Michaela ProcházkováPavel Plevka

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