Three-dimensional structure of the myosin V inhibited state by cryoelectron tomography.

Nature
Jun LiuKenneth A Taylor

Abstract

Unconventional myosin V (myoV) is an actin-based molecular motor that has a key function in organelle and mRNA transport, as well as in membrane trafficking. MyoV was the first member of the myosin superfamily shown to be processive, meaning that a single motor protein can 'walk' hand-over-hand along an actin filament for many steps before detaching. Full-length myoV has a low actin-activated MgATPase activity at low [Ca2+], whereas expressed constructs lacking the cargo-binding domain have a high activity regardless of [Ca2+] (refs 5-7). Hydrodynamic data and electron micrographs indicate that the active state is extended, whereas the inactive state is compact. Here we show the first three-dimensional structure of the myoV inactive state. Each myoV molecule consists of two heads that contain an amino-terminal motor domain followed by a lever arm that binds six calmodulins. The heads are followed by a coiled-coil dimerization domain (S2) and a carboxy-terminal globular cargo-binding domain. In the inactive structure, bending of myoV at the head-S2 junction places the cargo-binding domain near the motor domain's ATP-binding pocket, indicating that ATPase inhibition might occur through decreased rates of nucleotide exchange. The ...Continue Reading

References

Feb 1, 1991·Current Opinion in Cell Biology·J R Sellers
Apr 16, 1998·The Journal of Biological Chemistry·H L SweeneyJ R Sellers
Aug 17, 1999·Nature·A D MehtaR E Cheney
Sep 17, 1999·The Journal of Biological Chemistry·K M TrybusY Freyzon
Nov 13, 1999·Nature Cell Biology·D L CoyJ Howard
Dec 22, 1999·Journal of Structural Biology·S J LudtkeW Chiu
Feb 8, 2000·The Journal of Biological Chemistry·F WangJ R Sellers
Mar 21, 2000·Biochimica Et Biophysica Acta·S L Reck-PetersonJ A Mercer
May 12, 2000·Nature Cell Biology·D D Hackney, M F Stock
Aug 18, 2000·The Journal of Biological Chemistry·K HommaM Ikebe
Feb 19, 2002·Nature Neuroscience·Erin N StarVenkatesh N Murthy
Aug 2, 2003·Journal of Structural Biology·Hanspeter Winkler, Kenneth A Taylor
Nov 25, 2003·The Journal of Biological Chemistry·Fei WangJames R Sellers
Feb 21, 2004·Biochemical and Biophysical Research Communications·Xiang-dong LiMitsuo Ikebe
Mar 10, 2004·The Journal of Cell Biology·Dimitry N KrementsovKathleen M Trybus
Oct 29, 2004·The EMBO Journal·Pierre-Damien CoureuxAnne Houdusse

❮ Previous
Next ❯

Citations

Feb 2, 2008·Cellular and Molecular Life Sciences : CMLS·K M Trybus
Oct 13, 2010·Protoplasma·Jens TilsnerLesley Torrance
May 20, 2009·Cell Biochemistry and Biophysics·Wonmuk Hwang, Matthew J Lang
Dec 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Anne HoudusseCarolyn Cohen
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·M Yusuf AliDavid M Warshaw
Mar 22, 2007·Proceedings of the National Academy of Sciences of the United States of America·John A Hammer, Xufeng Wu
Dec 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Alexander HeuckDierk Niessing
Jan 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Xiang-Dong LiMitsuo Ikebe
Oct 14, 2009·Proceedings of the National Academy of Sciences of the United States of America·Erin M Craig, Heiner Linke
Jan 10, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jessica M ArmstrongDavid M Warshaw
Sep 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Qing LuMingjie Zhang
Jun 29, 2012·The Journal of Biological Chemistry·Motoki TominagaKazuhiro Oiwa
Apr 29, 2008·The Journal of Biological Chemistry·Neil M KadDavid M Warshaw
Sep 6, 2013·Molecular Biology of the Cell·Andrew J LindsayBruno Goud
Oct 12, 2010·Nucleic Acids Research·Catherine L LawsonWah Chiu
Jun 8, 2012·Computational and Mathematical Methods in Medicine·Zoe Katsimitsoulia, William R Taylor
Apr 29, 2009·BMC Bioinformatics·Carlos Oscar Sanchez SorzanoJ M Carazo
Dec 17, 2014·Biophysics Reviews·Noriyuki Kodera, Toshio Ando
Oct 10, 2013·Proceedings of the National Academy of Sciences of the United States of America·Shayantani Mukherjee, Arieh Warshel
Nov 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Olena PylypenkoAnne M Houdusse
May 5, 2010·The Journal of Cell Biology·Alexander HeuckDierk Niessing
Jun 5, 2012·Micron : the International Research and Review Journal for Microscopy·Jose-Jesus Fernandez
Jan 27, 2016·Proceedings of the National Academy of Sciences of the United States of America·Christopher BattersClaudia Veigel
Aug 21, 2010·Journal of Molecular Graphics & Modelling·William R Taylor, Zoe Katsimitsoulia
Jan 30, 2008·Current Opinion in Cell Biology·Jennifer L RossDavid M Warshaw
Jul 14, 2007·Journal of Molecular Biology·Jürgen BoschWim G J Hol
Jun 15, 2007·Journal of Structural Biology·Dianne W TaylorKenneth A Taylor
Jan 16, 2016·Journal of Biophysics·Arthur J MichalekM Yusuf Ali
Jan 9, 2007·Current Opinion in Cell Biology·Kenneth A Taylor
Dec 19, 2006·Current Opinion in Cell Biology·H Lee Sweeney, Anne Houdusse
Oct 14, 2014·Current Biology : CB·Li Xu, Anthony Bretscher
Mar 16, 2016·Proceedings of the National Academy of Sciences of the United States of America·Sarah F WulfRasmus R Schröder
Dec 26, 2006·Protein Science : a Publication of the Protein Society·Qinghua WangKylie J Walters
Jul 20, 2007·Biology of the Cell·Claire DesnosFrançois Darchen
Nov 2, 2013·Biology of the Cell·Andrew J Lindsay, Mary W McCaffrey
Jul 18, 2009·Cell Motility and the Cytoskeleton·Andrew J Lindsay, Mary W McCaffrey
Sep 16, 2006·Journal of Structural Biology·Hanspeter Winkler
Jan 1, 2012·Computational and Structural Biotechnology Journal·Mikhail KudryashevHenning Stahlberg

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