Three-dimensional structure of the porcine gastric H,K-ATPase from negatively stained crystals

Journal of Structural Biology
Y Xian, H Hebert

Abstract

A low-resolution three-dimensional model of membrane-bound H,K-ATPase from pig gastric mucosa has been reconstructed by electron microscopy and image processing of two-dimensional crystals in negative stain. The crystal formation is induced by magnesium and vanadate, which stabilize the E2 conformation of the enzyme. The unit cell, with a size of a = b = 123 A, gamma = 90 degrees, has tetragonal p4 symmetry. There are four separate alpha beta protomers within each unit cell. The high-contrast region is limited to the cytoplasmic part of the protein. The total volume of the observed asymmetric protein domain corresponds to a molecular mass of 80-90 kDa. It consists mainly of a large pear-shaped domain measuring 60 x 45 A2, with a height of 50 A as measured perpendicular to the membrane plane. A small stalk segment, 20 A in length, forms a connection to the transmembrane region.

References

Mar 9, 1992·FEBS Letters·H HebertS Mårdh
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·B H TohD C Humphris
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M A ReubenG Sachs
Dec 1, 1989·Journal of Ultrastructure and Molecular Structure Research·E SkriverW Schoner
Dec 11, 1987·Biochimica Et Biophysica Acta·M L Helmich-de JongJ J de Pont
Nov 30, 1988·Biochemical and Biophysical Research Communications·M MaedaM Futai
Jul 1, 1988·Journal of Ultrastructure and Molecular Structure Research·H HebertA B Maunsbach
Oct 1, 1985·Journal of Ultrastructure Research·M MohrazP R Smith
Aug 7, 1986·Biochimica Et Biophysica Acta·A SoumarmonM J Lewin
Oct 5, 1985·Journal of Molecular Biology·L CastellaniP Vibert
Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Aug 31, 1981·FEBS Letters·E SkriverP L Jørgensen
Aug 12, 1982·Biochimica Et Biophysica Acta·H HebertA B Maunsbach
Jan 11, 1984·Biochimica Et Biophysica Acta·M LjungströmS Mårdh
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Aug 1, 1982·Journal of Microscopy·W O Saxton, W Baumeister
Jan 1, 1982·Ultramicroscopy·R Hegerl, A Altbauer

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Citations

Oct 12, 2012·The Journal of Biological Chemistry·Ingrid DachChristine Ebel
Feb 16, 2008·Journal of Structural Biology·Tomohiro NishizawaYoshinori Fujiyoshi
Feb 25, 2003·Annual Review of Biophysics and Biomolecular Structure·David L Stokes, N Michael Green
Nov 11, 2005·Journal of Medicinal Chemistry·Chang G KimAnthony Watts
Sep 3, 1998·Biophysical Journal·J J LacapèreJ L Rigaud

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