Three-dimensional structures of the amyloid beta peptide (25-35) in membrane-mimicking environment

Biochemistry
T KohnoA Takashima

Abstract

The three-dimensional structure of amyloid beta peptide (25-35), which has neurotoxic activity, in lithium dodecyl sulfate micelles was determined by two-dimensional 1H NMR spectroscopy with simulated annealing calculations. A total of 20 converged amyloid beta peptide structures were obtained on the basis of 110 experimental constraints, including 106 distance constraints reduced from the nuclear Overhauser effect (NOE) connectivities and four torsion angle (phi) constraints. The atomic root mean square difference about averaged coordinates is 1.04 +/- 0.25 A for the backbone atoms (N, C alpha, C) and 1.39 +/- 0.27 A for all heavy atoms of the entire peptide. The molecular structure of amyloid beta peptide in membrane-mimicking environment is composed of a short alpha helix in the C terminal position. The three residues from the N-terminus are disordered, but the remaining eight C-terminal residues are well-ordered, which is supported by the RMSD values of the C-terminal region, Lys28-Leu34. In this region, the RMS differences from averaged coordinates are 0.26 +/- 0.11 A for the backbone atoms (N, C alpha, C) and 0.77 +/- 0.21 A for all heavy atoms, which is very low compared with those for the entire peptide. The four amino ...Continue Reading

References

Nov 1, 1992·Journal of Biomolecular NMR·M PiottoV Sklenár
Mar 5, 1991·Journal of Molecular Biology·C HilbichK Beyreuther
Dec 16, 1983·Biochemical and Biophysical Research Communications·M RanceK Wüthrich
Dec 1, 1994·Biophysical Journal·S R DurellH B Pollard
Oct 1, 1995·European Journal of Biochemistry·H StichtP Rösch
Oct 14, 1994·Biochemical and Biophysical Research Communications·T TomiyamaN Endo
Oct 1, 1993·Journal of Neurochemistry·J BusciglioB A Yankner
Jul 1, 1963·Journal of Molecular Biology·G N RAMACHANDRANV SASISEKHARAN

❮ Previous
Next ❯

Citations

May 4, 2006·European Biophysics Journal : EBJ·Silvia DanteNorbert A Dencher
Jul 28, 2007·Journal of Biomolecular NMR·Jüri JarvetAstrid Gräslund
Aug 1, 1997·Current Opinion in Structural Biology·H Mihara, Y Takahashi
Apr 25, 2012·Nature Reviews. Neurology·Maya Srikanth, John A Kessler
Jun 27, 2002·European Journal of Biochemistry·Andrea EnglerDieter Willbold
Dec 3, 2015·ACS Chemical Neuroscience·Natália E C de AlmeidaMichael T Bowers
Oct 7, 2004·Biopolymers·Ganesh Shanmugam, Rajadas Jayakumar
Oct 10, 2007·Journal of Peptide Science : an Official Publication of the European Peptide Society·Sylwia Rodziewicz-MotowidłoCezary Czaplewski
Nov 30, 2006·Journal of Neuroscience Research·Maria Laura GiuffridaEnrico Rizzarelli
Mar 8, 2013·Chemical Biology & Drug Design·Priya NarayanSrinivasarao Raghothama
Jul 14, 2010·Angewandte Chemie·Sara M Butterfield, Hilal A Lashuel
Aug 11, 2005·Biochemical and Biophysical Research Communications·Ganesh ShanmugamZsuzsa Majer
Jan 15, 2005·Progress in Neurobiology·Carlos MorganNibaldo C Inestrosa
Jan 24, 2006·Experimental Cell Research·Jordi MagraneHenry W Querfurth
Aug 23, 2012·Biochimica Et Biophysica Acta·Ganesh Shanmugam, Prasad L Polavarapu
Jul 16, 2002·Annual Review of Biomedical Engineering·Regina M Murphy
Jul 9, 2004·Biophysical Journal·Ganesh Shanmugam, Prasad L Polavarapu
Jul 19, 2000·Biochimica Et Biophysica Acta·L C Serpell
Aug 19, 2008·Biochimica Et Biophysica Acta·Gerardino D'ErricoAnna Maria D'Ursi
Jun 13, 2006·Biophysical Journal·Guanghong Wei, Joan-Emma Shea
Sep 1, 2017·Membranes·Adree KhondkerMaikel C Rheinstädter
Jun 29, 2001·Journal of Peptide Science : an Official Publication of the European Peptide Society·A B ClippingdaleC J Barrow
Mar 7, 2019·Protein and Peptide Letters·Dhandayuthapani SambasivamJayakumar Rajadas
Oct 19, 2017·The Journal of Physical Chemistry. B·Nabin KandelSuren A Tatulian
Oct 3, 2020·ACS Chemical Neuroscience·Elias KhayatAmy K Smith
Sep 30, 2021·Scientific Reports·Faisal AbedinSuren A Tatulian
Aug 6, 2004·Journal of Medicinal Chemistry·Anna M D'UrsiDelia Picone

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.