Three-dimensional synaptic organization of the human hippocampal CA1 field.

ELife
M Montero-CrespoL Blazquez-Llorca

Abstract

The hippocampal CA1 field integrates a wide variety of subcortical and cortical inputs, but its synaptic organization in humans is still unknown due to the difficulties involved studying the human brain via electron microscope techniques. However, we have shown that the 3D reconstruction method using Focused Ion Beam/Scanning Electron Microscopy (FIB/SEM) can be applied to study in detail the synaptic organization of the human brain obtained from autopsies, yielding excellent results. Using this technology, 24,752 synapses were fully reconstructed in CA1, revealing that most of them were excitatory, targeting dendritic spines and displaying a macular shape, regardless of the layer examined. However, remarkable differences were observed between layers. These data constitute the first extensive description of the synaptic organization of the neuropil of the human CA1 region.

References

Sep 15, 1992·The Journal of Comparative Neurology·R J MorecraftM M Mesulam
May 15, 1991·The Journal of Comparative Neurology·M P Witter, D G Amaral
Jan 1, 1991·Acta Neuropathologica·H Braak, E Braak
Aug 8, 1990·The Journal of Comparative Neurology·Y SamsonJ N Davis
Jul 22, 1988·The Journal of Comparative Neurology·R C Green, M M Mesulam
May 1, 1988·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·H J GundersenA Vesterby
Jan 15, 1986·The Journal of Comparative Neurology·J P AggletonM Mishkin
Jan 1, 1986·Experimental Brain Research·J P Aggleton
Feb 15, 1980·The Journal of Comparative Neurology·D G Amaral, W M Cowan
Jul 15, 1994·The Journal of Comparative Neurology·S De LacalleC B Saper
Dec 25, 1995·The Journal of Comparative Neurology·S T Carmichael, J L Price
Sep 23, 1996·The Journal of Comparative Neurology·K D Micheva, C Beaulieu
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·T Schikorski, C F Stevens
Jun 24, 1999·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·V KlimekG A Ordway
Dec 22, 1999·Journal of Neurophysiology·H Dvorak-Carbone, E M Schuman
Mar 24, 2000·Cerebral Cortex·C CavadaF Reinoso-Suárez
Mar 2, 2005·PLoS Biology·Sen SongDmitri B Chklovskii
Nov 26, 2005·The European Journal of Neuroscience·John P AggletonRichard C Saunders

❮ Previous
Next ❯

Citations

Sep 6, 2020·Cerebral Cortex·M Domínguez-ÁlvaroL Alonso-Nanclares
Dec 17, 2020·Brain : a Journal of Neurology·Marta Montero-CrespoLidia Blazquez-Llorca
May 30, 2021·Nature Reviews. Neuroscience·Haruo KasaiTaro Toyoizumi
May 18, 2021·Cerebral Cortex·Nicolás Cano-AstorgaLidia Alonso-Nanclares

❮ Previous
Next ❯

Methods Mentioned

BETA
light
electron microscopy

Software Mentioned

Stereo Investigator
Easyfit Proffessional
R
Spatstat package
GraphPad
EspINA Interactive Neuron Analyzer
Image J Stereology Toolset
ImageJ
SPSS Statistics
MathWave

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

BioRxiv : the Preprint Server for Biology
M Montero-CrespoL Blazquez-Llorca
BioRxiv : the Preprint Server for Biology
N. Cano-AstorgaL Alonso-Nanclares
BioRxiv : the Preprint Server for Biology
M Domínguez-ÁlvaroL Alonso-Nanclares
© 2021 Meta ULC. All rights reserved