Three-dimensional system enabling the maintenance and directed differentiation of pluripotent stem cells under defined conditions

Science Advances
Denise ZujurShinsuke Ohba

Abstract

The development of in vitro models for the maintenance and differentiation of pluripotent stem cells (PSCs) is an active area of stem cell research. The strategies used so far are based mainly on two-dimensional (2D) cultures, in which cellular phenotypes are regulated by soluble factors. We show that a 3D culture system with atelocollagen porous scaffolds can significantly improve the outcome of the current platforms intended for the maintenance and lineage specification of mouse PSCs (mPSCs). Unlike 2D conditions, the 3D conditions maintained the undifferentiated state of mouse embryonic stem cells (mESCs) without exogenous stimulation and also supported endoderm, mesoderm, and ectoderm differentiation of mESCs under serum-free conditions. Moreover, 3D mPSC-derived mesodermal cells showed accelerated osteogenic differentiation, giving rise to functional osteoblast-osteocyte populations within calcified structures. The present strategy offers a 3D platform suitable for the formation of organoids that mimic in vivo organs containing various cell types, and it may be adaptable to the generation of ectoderm-, mesoderm-, and endoderm-derived tissues when combined with appropriate differentiation treatments.

References

Nov 1, 1988·Endocrinology·N TakahashiT Suda
Feb 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Z BogdanovicA C Lichtler
Jan 5, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·I KalajzicD Rowe
Sep 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Gregory N BancroftAntonios G Mikos
Dec 13, 2003·Tissue Engineering·Sarah H CartmellRobert E Guldberg
Mar 26, 2004·Methods in Molecular Biology·Gisele HöpflIsabelle Desbaillets
Jul 2, 2004·Nature Biotechnology·Sheng Ding, Peter G Schultz
Jun 8, 2006·Journal of Biomedical Materials Research. Part a·Benjamin L BecksteadCecilia M Giachelli
Nov 24, 2006·Advances in Experimental Medicine and Biology·Heidi L HoltorfAntonios G Mikos
Feb 24, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shinsuke OhbaUng-il Chung
Nov 9, 2007·Gene Therapy·R C SchugarB M Deasy
Jan 29, 2008·Scandinavian Journal of Clinical and Laboratory Investigation·X-F TianT Cao
May 24, 2008·Nature·Qi-Long YingAustin Smith
Jul 8, 2008·Tissue Engineering. Part a·Elias VolkmerMatthias Schieker
Nov 10, 2009·Trends in Pharmacological Sciences·Wenlin Li, Sheng Ding
Jun 16, 2010·Journal of Bioscience and Bioengineering·Takako TakitohMasayuki Hara
Sep 30, 2010·Journal of Tissue Engineering and Regenerative Medicine·Jose F Alvarez-BarretoVassilios I Sikavitsas
Nov 3, 2010·Methods in Molecular Biology·Lee ButteryKevin Shakesheff
Sep 1, 2011·Nature Methods·Thomas P KraehenbuehlLino S Ferreira
Sep 13, 2011·Nature Medicine·Jinhu XiongCharles A O'Brien
Sep 13, 2011·Nature Medicine·Tomoki NakashimaHiroshi Takayanagi
May 1, 2012·Cell·Hendrik MarksHendrik G Stunnenberg
Feb 27, 2014·SpringerPlus·Xuan MengJiahong Dong
Aug 15, 2014·BoneKEy Reports·Brya G MatthewsJillian Cornish
Jan 12, 2016·Nature Materials·Massimiliano CaiazzoMatthias P Lutolf
Sep 3, 2016·Experimental & Molecular Medicine·Young Jae MoonEui-Sic Cho

❮ Previous
Next ❯

Citations

Aug 14, 2020·Experimental & Molecular Medicine·Shoichiro TaniHironori Hojo
Aug 16, 2017·Nanoscale Research Letters·Elena PavlovichAnatoliy Goltsev
Jun 12, 2019·Nature Biomedical Engineering·Shunsuke KawaiJunya Toguchida
Jun 22, 2019·Nature Reviews. Materials·Lorenzo MoroniJames J Yoo
Nov 12, 2019·Frontiers in Bioengineering and Biotechnology·Jeonghyun Kim, Taiji Adachi
Dec 29, 2019·Scientific Reports·Jennifer LeeJi Hyeon Ju
Jan 8, 2021·Soft Matter·Laura K Rivera-TarazonaTaylor H Ware
Feb 13, 2021·International Journal of Molecular Sciences·Shoichiro TaniHironori Hojo
Feb 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Sara Borrego-GonzálezRick Visser
Jun 13, 2020·Stem Cell Reports·Shoko OnoderaShinsuke Ohba
Jul 2, 2021·Frontiers in Bioengineering and Biotechnology·Ya An TsaiShinji Takeoka
Nov 12, 2018·ACS Biomaterials Science & Engineering·Carley OrtKibret Mequanint
Feb 25, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Lauren TomlinsonParveen Sharma

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence microscopy
scanning electron microscopy

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.