Three-Dimensional Tissue Models for Studying Ex Vivo Megakaryocytopoiesis and Platelet Production

Methods in Molecular Biology
C A Di BuduoA Balduini

Abstract

Three-dimensional (3D) tissue cultures in vitro enable a more physiological reconstruction of native tissues and organs. The bone marrow environment, structure and composition regulate megakaryocyte function and platelet production. Here, we describe the use of silk fibroin protein biomaterials to assemble 3D scaffolds mimicking the bone marrow niche architecture and extracellular matrix composition to support platelet release from human megakaryocytes. Additionally, we also propose the use of hyaluronan hydrogels, functionalized with extracellular matrix components, to reproduce the 3D matrix structure of the bone marrow environment for studying human megakaryocyte function.

Citations

Jun 12, 2020·Platelets·Vittorio AbbonanteAlessandra Balduini
Nov 25, 2020·Pharmaceutics·Alessandra GirottiFrancisco Javier Arias
Sep 22, 2021·Blood Advances·Vittorio AbbonanteAlessandra Balduini

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