Three-Dimensional Two-Color Dual-Particle Tracking Microscope for Monitoring DNA Conformational Changes and Nanoparticle Landings on Live Cells

ACS Nano
Yen-Liang LiuHsin-Chih Yeh

Abstract

Here, we present a three-dimensional two-color dual-particle tracking (3D-2C-DPT) technique that can simultaneously localize two spectrally distinct targets in three dimensions with a time resolution down to 5 ms. The dual-targets can be tracked with separation distances from 33 to 250 nm with tracking precisions of ∼15 nm (for static targets) and ∼35 nm (for freely diffusing targets). Since each target is individually localized, a wealth of data can be extracted, such as the relative 3D position, the 2D rotation, and the separation distance between the two targets. Using this technique, we turn a double-stranded DNA (dsDNA)-linked dumbbell-like dimer into a nanoscopic optical ruler to quantify the bending dynamics of nicked or gapped dsDNA molecules in free solution by manipulating the design of dsDNA linkers (1-nick, 3-nt, 6-nt, or 9-nt single-strand gap), and the results show the increase of kon (linear to bent) from 3.2 to 10.7 s-1. The 3D-2C-DPT is then applied to observe translational and rotational motions of the landing of an antibody-conjugated nanoparticle on the plasma membrane of living cells, revealing the reduction of rotations possibly due to interactions with membrane receptors. This study demonstrates that this...Continue Reading

References

Jan 1, 1978·Annual Review of Biochemistry·L Stryer
Jun 1, 1971·The Review of Scientific Instruments·H C Berg
Aug 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·L Stryer, R P Haugland
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·T HaS Weiss
Jan 1, 1997·Annual Review of Biophysics and Biomolecular Structure·M J Saxton, K Jacobson
Jun 17, 2000·Science·X ZhuangS Chu
Jun 12, 2002·The Journal of Cell Biology·Takahiro FujiwaraAkihiro Kusumi
Sep 27, 2002·Biophysical Journal·Douglas S MartinJosef A Käs
Jul 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Melike LakadamyaliXiaowei Zhuang
Sep 25, 2003·Biochemical Society Transactions·V LeviE Gratton
Jun 19, 2004·Science·Yaron Shav-TalRobert H Singer
Sep 3, 2004·Journal of Molecular Biology·Ekaterina ProtozanovaMaxim D Frank-Kamenetskii
Mar 3, 2005·Journal of the American Chemical Society·C S YunG F Strouse
May 24, 2005·Nature Biotechnology·Carsten SönnichsenA Paul Alivisatos
Aug 8, 2006·Biophysical Journal·Porntula PanorchanDenis Wirtz
Sep 23, 2006·Nature Methods·Nathalie ArhelPierre Charneau
Nov 17, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Stephen M AnthonySteve Granick
Oct 13, 2007·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Marco G MazzaFrancis W Starr
Jul 22, 2008·Nature Cell Biology·Nicholas L AndrewsDiane S Lidke
Aug 30, 2008·Journal of the Royal Society, Interface·Christian Hellriegel, Enrico Gratton
Sep 3, 2007·Optics Express·Ki Hean KimPeter T C So
Nov 3, 2009·Nature Methods·Philipp KukuraVahid Sandoghdar
Mar 31, 2010·Proceedings of the National Academy of Sciences of the United States of America·Steffen J SahlChristian Eggeling
May 19, 2010·Quarterly Reviews of Biophysics·Justin P Peters, L James Maher
Oct 21, 2010·Nano Letters·Nathan P WellsJames H Werner
Sep 22, 2011·Optics Express·Gary L HunterEric R Weeks
Oct 17, 2012·Proceedings of the National Academy of Sciences of the United States of America·Kazem V EdmondEric R Weeks
Nov 2, 2013·Nature Communications·William E UspalPatrick S Doyle
Dec 7, 2013·Journal of the American Chemical Society·Yuan Gao, Yan Yu
Jan 25, 2014·Science·Hye Yoon ParkRobert H Singer
Mar 25, 2014·Biophysical Journal·Jan-Willem van de MeentRuben L Gonzalez
Apr 5, 2014·Journal of Biomedical Optics·Xiao YuMyung K Kim
May 31, 2014·Science·Nikta FakhriChristoph F Schmidt
Jun 18, 2014·Nature Chemical Biology·Akihiro KusumiTakahiro K Fujiwara

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Citations

Apr 23, 2021·Physical Chemistry Chemical Physics : PCCP·Zhihong ZhangKai Yang
Aug 10, 2021·Current Opinion in Biomedical Engineering·Yuan-I ChenHsin-Chih Yeh

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