Three-dimensional ultrastructural imaging reveals the nanoscale architecture of mammalian cells

IUCrJ
Shengkun YaoHuaidong Jiang

Abstract

Knowledge of the interactions between nanomaterials and large-size mammalian cells, including cellular uptake, intracellular localization and translocation, has greatly advanced nanomedicine and nanotoxicology. Imaging techniques that can locate nanomaterials within the structures of intact large-size cells at nanoscale resolution play crucial roles in acquiring this knowledge. Here, the quantitative imaging of intracellular nanomaterials in three dimensions was performed by combining dual-energy contrast X-ray microscopy and an iterative tomographic algorithm termed equally sloped tomography (EST). Macrophages with a size of ∼20 µm that had been exposed to the potential antitumour agent [Gd@C82(OH)22] n were investigated. Large numbers of nanoparticles (NPs) aggregated within the cell and were mainly located in phagosomes. No NPs were observed in the nucleus. Imaging of the nanomedicine within whole cells advanced the understanding of the high-efficiency antitumour activity and the low toxicity of this agent. This imaging technique can be used to probe nanomaterials within intact large-size cells at nanometre resolution uniformly in three dimensions and may greatly benefit the fields of nanomedicine and nanotoxicology.

References

Jul 1, 1979·Investigative Radiology·T L HoukD C Flemming
Jul 1, 1989·Ultrastructural Pathology·J M Papadimitriou, R B Ashman
Feb 1, 1995·Quarterly Reviews of Biophysics·J KirzM Howells
Jul 1, 1997·Physics in Medicine and Biology·R Lewis
Apr 5, 2003·Science·David J Stephens, Victoria J Allan
Oct 2, 2003·Nature Biotechnology·Vicki L Colvin
Nov 5, 2003·Nature Biotechnology·Stefan W Hell
Jan 18, 2005·Trends in Cell Biology·Richard McIntoshDavid Mastronarde
Jul 9, 2005·Environmental Health Perspectives·Günter OberdörsterJan Oberdörster
Sep 13, 2005·Current Opinion in Structural Biology·Mark A Le GrosCarolyn A Larabell
Aug 12, 2006·Science·Eric BetzigHarald F Hess
Aug 25, 2007·Journal of Synchrotron Radiation·Göran A JohanssonAdam P Hitchcock
Dec 23, 2008·Trends in Biochemical Sciences·Andrew LeisWolfgang Baumeister
Jun 6, 2009·Annual Review of Biochemistry·Bo HuangXiaowei Zhuang
Nov 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Maho UchidaCarolyn A Larabell
Apr 7, 2010·Proceedings of the National Academy of Sciences of the United States of America·Xing-Jie LiangLee Jia
Apr 8, 2010·Cell·Bin-Zhi Qian, Jeffrey W Pollard
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Huaidong JiangJianwei Miao
Aug 3, 2010·Nature Methods·Vasilis Ntziachristos
Aug 20, 2010·Proceedings of the National Academy of Sciences of the United States of America·Martin D de JongeStefan Vogt
Sep 28, 2010·Current Opinion in Structural Biology·Carolyn A Larabell, Keith A Nugent
Oct 5, 2010·Chemical Society Reviews·Leo Y T ChouWarren C W Chan
Oct 12, 2010·Current Opinion in Structural Biology·Martin D de Jonge, Stefan Vogt
Nov 16, 2010·Nature Methods·Gerd SchneiderJames G McNally
Dec 3, 2010·Journal of Nanoscience and Nanotechnology·Jie MengXing-Jie Liang
Jan 27, 2012·Trends in Immunology·Brian RuffellLisa M Coussens
Feb 1, 2012·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Gerry McDermottCarolyn A Larabell
Mar 23, 2012·Nature·M C ScottJianwei Miao
Sep 11, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Jie MengYuliang Zhao
Oct 24, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yunzhe ZhaoAlberto Bravin
Mar 28, 2013·Nanoscale·Liang-Chien ChengRu-Shi Liu
Aug 8, 2013·Microscopy Research and Technique·Thomas L KaoWenge Yang
Sep 17, 2013·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Joy C Andrews, Bert M Weckhuysen
Oct 24, 2013·Nature Materials·Vikash P Chauhan, Rakesh K Jain
Aug 1, 1987·Biological Trace Element Research·H RarbackR Rosser
Jan 8, 2014·Trends in Plant Science·Fang-Jie ZhaoYong-Guan Zhu
Jan 24, 2014·Nature Materials·Ralph WeisslederMikael J Pittet
Apr 23, 2014·Applied Physics Letters·Yijin LiuWenge Yang

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray
light microscopy
electron microscopy

Software Mentioned

Amira
STXM
PPFFT

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.