Three-dimensional ultrastructure of Plasmodium falciparum throughout cytokinesis.

PLoS Pathogens
Rachel M RudlaffJeffrey D Dvorin

Abstract

New techniques for obtaining electron microscopy data through the cell volume are being increasingly utilized to answer cell biologic questions. Here, we present a three-dimensional atlas of Plasmodium falciparum ultrastructure throughout parasite cell division. Multiple wild type schizonts at different stages of segmentation, or budding, were imaged and rendered, and the 3D structure of their organelles and daughter cells are shown. Our high-resolution volume electron microscopy both confirms previously described features in 3D and adds new layers to our understanding of Plasmodium nuclear division. Interestingly, we demonstrate asynchrony of the final nuclear division, a process that had previously been reported as synchronous. Use of volume electron microscopy techniques for biological imaging is gaining prominence, and there is much we can learn from applying them to answer questions about Plasmodium cell biology. We provide this resource to encourage readers to consider adding these techniques to their cell biology toolbox.

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Citations

Nov 17, 2020·Cellular Microbiology·Tania F de Koning-WardFreya J I Fowkes
Dec 16, 2020·Parasitology International·Ying ZhangHonglin Jia
Jan 26, 2021·Frontiers in Cellular and Infection Microbiology·Josie Liane FerreiraTim-Wolf Gilberger
Apr 27, 2021·Frontiers in Cellular and Infection Microbiology·Marta MachadoMarkus Ganter
Apr 30, 2021·Frontiers in Cellular and Infection Microbiology·Marc-Jan GubbelsKlemens Engelberg
May 7, 2021·Frontiers in Cellular and Infection Microbiology·Alexander A Morano, Jeffrey D Dvorin
Aug 25, 2021·MBio·Julie M J VerhoefTaco W A Kooij

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Methods Mentioned

BETA
electron microscopy
fluorescence microscopy
electron
electron tomography
super-resolution
transgenic
imaging techniques

Software Mentioned

ImageJ
Helios
Avizo
Matlab
Atlas
EMPIAR

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