Three-dimensional visualization of in vitro cultivated chondrocytes inside porous gelatine scaffolds: A tomographic approach

Acta Biomaterialia
Rolf ZehbeHelmut Schubert

Abstract

Synchrotron radiation-based microcomputed tomography (SR-microCT) has become a valuable tool in the structural characterization of different types of materials, achieving volumetric details with micrometre resolution. Biomedical research dealing with porous polymeric biomaterials is one of the research fields which can benefit greatly from the use of SR-microCT. This study demonstrates that current experimental set-ups at synchrotron beamlines achieve a sufficiently high resolution in order to visualize the positions of individual cartilage cells cultivated on porous gelatine scaffolds made by a freeze-structuring technique. Depending on the processing parameters, the pore morphology of the scaffolds investigated was changed from large-pore sized but non-ordered structures to highly directional and fine pored. The cell-seeded scaffolds were stained with a combined Au/Ag stain to enhance the absorption contrast in SR-microCT. While only some cells showed enhanced absorption contrast, most cells did not show any difference in contrast to the surrounding scaffold and were consequently not detectable using conventional greyscale threshold methods. Therefore, using an image-based three-dimensional segmentation tool on the tomographi...Continue Reading

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Citations

Feb 4, 2012·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Brian M PattersonEllen Cerreta
Jun 21, 2011·Tissue Engineering. Part B, Reviews·Alyssa AppelEric M Brey
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May 30, 2013·Tissue Engineering. Part C, Methods·Zohreh IzadifarXiongbiao Chen
Feb 13, 2014·Tissue Engineering. Part B, Reviews·Adeola Deborah OlubamijiDaniel Xiongbiao Chen
Mar 10, 2016·Materials Science & Engineering. C, Materials for Biological Applications·A ThiemR Zehbe
May 29, 2012·Micron : the International Research and Review Journal for Microscopy·Rolf ZehbeChristoph Brochhausen
Jun 19, 2013·Biomaterials·Alyssa A AppelEric M Brey
Jun 12, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Rolf Zehbe, Kerstin Zehbe
Nov 8, 2012·The Journal of Physical Chemistry. B·Carolina MochalesWolf-Dieter Mueller
Sep 17, 2016·Macromolecular Bioscience·Robert S BradleyMohammed Yusuf
Dec 18, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Kankan QinFrancisco M Fernandes
Aug 3, 2021·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Cleo ParisiFrancisco M Fernandes

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